Expression of collagen types i and II on articular cartilage in a rat knee contracture model

Yoshihiro Hagiwara, Akira Ando, Eiichi Chimoto, Masahiro Tsuchiya, Ichiro Takahashi, Yasuyuki Sasano, Yoshito Onoda, Hideaki Suda, Eiji Itoi

Research output: Contribution to journalArticle

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Abstract

The purpose of our study was to clarify the expression patterns of collagen types I and II on articular cartilage after immobilization in a rat knee contracture model in 3 specific areas (noncontact area, transitional area, contact area). The unilateral knee joints of adult male rats were rigidly immobilized at 150° of flexion using screws and a rigid plastic plate. Sham-operated animals had holes drilled in the femur and the tibia and screws inserted but were not plated. The expression patterns of collagen types I and II in each area were evaluated by in situ hybridization (ISH), immunohistochemistry (IHC), and quantitative real-time polymerase chain reaction (qPCR). The expression of collagen type II in the noncontact area was decreased by ISH but appeared unchanged when examined by IHC. In the transitional and contact areas, the expression of collagen type II was initially shown to have decreased and then increased at the hypertrophic chondrocytes by ISH but appeared decreased by IHC. Quantitative PCR revealed the decreased expression of type II collagen in the contact area. Immunostaining of collagen type I was increased at the noncontact area and transitional areas. Alterations of collagen types I and II expression may also affect the degeneration of articular cartilage after immobilization and the changes were different in the three areas.

Original languageEnglish
Pages (from-to)22-30
Number of pages9
JournalConnective Tissue Research
Volume51
Issue number1
DOIs
Publication statusPublished - Feb 1 2010

Fingerprint

Collagen Type II
Cartilage
Articular Cartilage
Contracture
Rats
Knee
Collagen
Collagen Type I
In Situ Hybridization
Immunohistochemistry
Immobilization
Polymerase chain reaction
Chondrocytes
Knee Joint
Tibia
Femur
Plastics
Real-Time Polymerase Chain Reaction
Animals
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Expression of collagen types i and II on articular cartilage in a rat knee contracture model. / Hagiwara, Yoshihiro; Ando, Akira; Chimoto, Eiichi; Tsuchiya, Masahiro; Takahashi, Ichiro; Sasano, Yasuyuki; Onoda, Yoshito; Suda, Hideaki; Itoi, Eiji.

In: Connective Tissue Research, Vol. 51, No. 1, 01.02.2010, p. 22-30.

Research output: Contribution to journalArticle

Hagiwara, Y, Ando, A, Chimoto, E, Tsuchiya, M, Takahashi, I, Sasano, Y, Onoda, Y, Suda, H & Itoi, E 2010, 'Expression of collagen types i and II on articular cartilage in a rat knee contracture model', Connective Tissue Research, vol. 51, no. 1, pp. 22-30. https://doi.org/10.3109/03008200902859406
Hagiwara, Yoshihiro ; Ando, Akira ; Chimoto, Eiichi ; Tsuchiya, Masahiro ; Takahashi, Ichiro ; Sasano, Yasuyuki ; Onoda, Yoshito ; Suda, Hideaki ; Itoi, Eiji. / Expression of collagen types i and II on articular cartilage in a rat knee contracture model. In: Connective Tissue Research. 2010 ; Vol. 51, No. 1. pp. 22-30.
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