Expression of C3 and C4 photosynthetic characteristics in the amphibious plant Eleocharis vivipara

Structure and analysis of the expression of isogenes for pyruvate, orthophosphate dikinase

Sakae Agarie, Motoshi Kai, Hiroshi Takatsuji, Ueno Osamu

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Eleocharis vivipara, a unique leafless amphibious sedge, adopts the C4 mode of photosynthesis under terrestrial conditions and the C3 mode under submerged aquatic conditions. To analyze the molecular basis of these responses to the contrasting environments, we isolated and characterized two full-length cDNAs for a key C4 enzyme, pyruvate, orthophosphate dikinase (PPDK; EC 2.7.9.1). The isogenes for PPDK, designated ppdk1 and ppdk2, were highly homologous to one another but not identical. The PPDK1 protein, deduced from the nucleotide sequence of the cDNA, contained an extra domain at the amino terminus which, presumably, serves as a chloroplast transit peptide, while PPDK2 lacked this extra domain. It seems likely, therefore, that the ppdk1 and ppdk2 genes encode a chloroplastic and a cytosolic PPDK, respectively. Genomic Southern blot analysis revealed the existence of a small family of genes for PPDK in the genome of E. vivipara. Northern blot analysis indicate that both chloroplastic and cytosolic genes for PPDK are expressed simultaneously in the culms, a photosynthetic organ, of E, vivipara and that the pattern of expression of these genes differs between the growth forms.

Original languageEnglish
Pages (from-to)363-369
Number of pages7
JournalPlant Molecular Biology
Volume34
Issue number2
DOIs
Publication statusPublished - Jan 1 1997
Externally publishedYes

Fingerprint

Eleocharis
Orthophosphate Dikinase Pyruvate
Complementary DNA
Genes
genes
Photosynthesis
Southern Blotting
Northern blotting
Northern Blotting
Southern blotting
chloroplasts
photosynthesis
Genome
peptides
genomics
Gene Expression
nucleotide sequences
gene expression
genome
Enzymes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Expression of C3 and C4 photosynthetic characteristics in the amphibious plant Eleocharis vivipara : Structure and analysis of the expression of isogenes for pyruvate, orthophosphate dikinase. / Agarie, Sakae; Kai, Motoshi; Takatsuji, Hiroshi; Osamu, Ueno.

In: Plant Molecular Biology, Vol. 34, No. 2, 01.01.1997, p. 363-369.

Research output: Contribution to journalArticle

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abstract = "Eleocharis vivipara, a unique leafless amphibious sedge, adopts the C4 mode of photosynthesis under terrestrial conditions and the C3 mode under submerged aquatic conditions. To analyze the molecular basis of these responses to the contrasting environments, we isolated and characterized two full-length cDNAs for a key C4 enzyme, pyruvate, orthophosphate dikinase (PPDK; EC 2.7.9.1). The isogenes for PPDK, designated ppdk1 and ppdk2, were highly homologous to one another but not identical. The PPDK1 protein, deduced from the nucleotide sequence of the cDNA, contained an extra domain at the amino terminus which, presumably, serves as a chloroplast transit peptide, while PPDK2 lacked this extra domain. It seems likely, therefore, that the ppdk1 and ppdk2 genes encode a chloroplastic and a cytosolic PPDK, respectively. Genomic Southern blot analysis revealed the existence of a small family of genes for PPDK in the genome of E. vivipara. Northern blot analysis indicate that both chloroplastic and cytosolic genes for PPDK are expressed simultaneously in the culms, a photosynthetic organ, of E, vivipara and that the pattern of expression of these genes differs between the growth forms.",
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