Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes

Yoshikazu Higami, Tomoshi Tsuchiya, Kazuo To, Takuya Chiba, Haruyoshi Yamaza, Daisuke Shiokawa, Sei Ichi Tanuma, Isao Shimokawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase y was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). DNase y immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase y immunoreactivity was not detected during LPS-induced or CCl4-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase y plays an important role in Fasindependent apoptotic DNA fragmentation in hepatocytes.

Original languageEnglish
Pages (from-to)403-407
Number of pages5
JournalCell and Tissue Research
Volume316
Issue number3
DOIs
Publication statusPublished - Jun 2004
Externally publishedYes

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Deoxyribonucleases
DNA Fragmentation
Hepatocytes
Rodentia
DNA
Endonucleases
Cycloheximide
Labeling
Lipopolysaccharides
Necrosis
Apoptosis
Carbon Tetrachloride
Liver
Paraffin
Immunohistochemistry
deoxyribonuclease gamma
Antibodies

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes. / Higami, Yoshikazu; Tsuchiya, Tomoshi; To, Kazuo; Chiba, Takuya; Yamaza, Haruyoshi; Shiokawa, Daisuke; Tanuma, Sei Ichi; Shimokawa, Isao.

In: Cell and Tissue Research, Vol. 316, No. 3, 06.2004, p. 403-407.

Research output: Contribution to journalArticle

Higami, Y, Tsuchiya, T, To, K, Chiba, T, Yamaza, H, Shiokawa, D, Tanuma, SI & Shimokawa, I 2004, 'Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes', Cell and Tissue Research, vol. 316, no. 3, pp. 403-407. https://doi.org/10.1007/s00441-004-0890-x
Higami, Yoshikazu ; Tsuchiya, Tomoshi ; To, Kazuo ; Chiba, Takuya ; Yamaza, Haruyoshi ; Shiokawa, Daisuke ; Tanuma, Sei Ichi ; Shimokawa, Isao. / Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes. In: Cell and Tissue Research. 2004 ; Vol. 316, No. 3. pp. 403-407.
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abstract = "Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase y was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). DNase y immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase y immunoreactivity was not detected during LPS-induced or CCl4-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase y plays an important role in Fasindependent apoptotic DNA fragmentation in hepatocytes.",
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AU - Higami, Yoshikazu

AU - Tsuchiya, Tomoshi

AU - To, Kazuo

AU - Chiba, Takuya

AU - Yamaza, Haruyoshi

AU - Shiokawa, Daisuke

AU - Tanuma, Sei Ichi

AU - Shimokawa, Isao

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N2 - Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase y was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). DNase y immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase y immunoreactivity was not detected during LPS-induced or CCl4-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase y plays an important role in Fasindependent apoptotic DNA fragmentation in hepatocytes.

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