Expression of glutamate transporter subtypes during normal human corticogenesis and type II lissencephaly

Akiko Furuta, Sachio Takashima, Hideaki Yokoo, Jeffrey D. Rothstein, Keiji Wada, Toru Iwaki

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Glutamate transporters are thought to have an important role in central nervous system (CNS) development. We investigated the expression of the sodium-dependent high-affinity glutamate transporters EAAT1, EAAT2, and EAAT3 in 11 human autopsied cases without neurological disorders and in four cases with type II lissencephaly including Walker Warburg's syndrome (WWS) and Fukuyama-type congenital muscular dystrophy (FCMD), both of which are classified as migration disorders of the human brain. Expression of glutamate transporter subtypes was differentially regulated during normal human corticogenesis. Although EAAT1 and EAAT2 were mainly localized to the cortical astrocytes in the postnatal brain, EAAT1 was enriched in the proliferative zones and radial glia from 13 gestational weeks (GW) to 20 GW. EAAT2 was abundant in the intermediate zone until 23 GW, and transiently expressed in the radial fibers of the transitional form of radial glia into mature astrocytes as well as partly in the corticofugal axonal bundles. EAAT3 immunoreactivity was robust in the apical dendrites of the pyramidal neurons in the marginal zone and cortical plate during corticogenesis, and decreased postnatally. In the individuals with type II lissencephaly, glutamate transporters were expressed in the extrusion of neuroglial tissue. Bundles of EAAT2-immunoreactive radial fibers were prominent in the specimens at 20 GW. Thus, glutamate transporters are differentially regulated during normal and impaired corticogenesis. Altered glutamate transporter expression in type II lissencephaly suggests that glutamate metabolism is involved in the formation of the normal cortex and contributes to the disorganized cortex seen in migration disorders.

Original languageEnglish
Pages (from-to)155-164
Number of pages10
JournalDevelopmental Brain Research
Volume155
Issue number2
DOIs
Publication statusPublished - Mar 31 2005

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Lissencephaly
Amino Acid Transport System X-AG
Walker-Warburg Syndrome
Neuroglia
Astrocytes
Pyramidal Cells
Brain Diseases
Dendrites
Nervous System Diseases
Cerebral Cortex
Glutamic Acid
Central Nervous System
Sodium
Brain

All Science Journal Classification (ASJC) codes

  • Developmental Neuroscience
  • Developmental Biology

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Expression of glutamate transporter subtypes during normal human corticogenesis and type II lissencephaly. / Furuta, Akiko; Takashima, Sachio; Yokoo, Hideaki; Rothstein, Jeffrey D.; Wada, Keiji; Iwaki, Toru.

In: Developmental Brain Research, Vol. 155, No. 2, 31.03.2005, p. 155-164.

Research output: Contribution to journalArticle

Furuta, Akiko ; Takashima, Sachio ; Yokoo, Hideaki ; Rothstein, Jeffrey D. ; Wada, Keiji ; Iwaki, Toru. / Expression of glutamate transporter subtypes during normal human corticogenesis and type II lissencephaly. In: Developmental Brain Research. 2005 ; Vol. 155, No. 2. pp. 155-164.
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