Expression of hepatocyte growth factor gene in endothelial and kupffer cells of damaged rat livers, as revealed by in situ hydridization

Sumihara Noji, Kosuke Tashiro, Eiki Koyama, Tsutomu Nohno, Kazuhiko Ohyama, Shigehiko Taniguchi, Toshikazu Nakamura

Research output: Contribution to journalArticle

274 Citations (Scopus)

Abstract

Hepatocyte growth factor (HGP) has been demonstrated to be synthesized and secreted by non-parenchymal liver cells for liver regeneration after hepatic injury. We performed in situ hybridization to identify HGF-producing cell types in rat liver hepatitis induced by administrating carbon tetrachloride as a hepatotoxin. We found that transcripts of the HGF gene are localized in the Kupffer and endothelial cells in normal livers and increased remarkably in the Kupffer cells of the damaged livers. Thus, HGF is concluded to be synthesized in the Kupffer and endothelial cells to repair the liver tissue in paracrine fashion. No significant increase in the transcripts of the HGF gene was observed in livers after partial hepatectomy, indicating that a mehcanism on liver regeneration after the hepatectomy differs from that on liver repairs. Since the HGF gene expression was also found in lung and kidney, HGF may be a ubiquitous factor for tissue repairs.

Original languageEnglish
Pages (from-to)42-47
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume173
Issue number1
DOIs
Publication statusPublished - Nov 30 1990

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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