Expression of Id and ITF-2 genes in the mammary gland during pregnancy

Yoko Itahana, Marie Piens, Sylvia Fong, Jarnail Singh, Tomoki Sumida, Pierre Yves Desprez

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Id-1 is one of the four related helix-loop-helix Id proteins that act as inhibitors of the basic helix-loop-helix (bHLH) transcription factors that control cell differentiation, development and carcinogenesis. We previously found that Id-1 regulated the growth, differentiation, apoptosis and invasion of mouse mammary epithelial cells in culture. Using the techniques of immunohistochemistry and in situ hybridization, we now show that Id-1 gene expression is specifically detected in the epithelial cells of growing ductal structures during early pregnancy. Using adjacent sections, we determined that Id-1 was expressed in keratin 8/18 positive cells. We also demonstrated that the up-regulation of Id-2 during late pregnancy correlated with the down-regulation of Id-1. Using the yeast-two hybrid system, we identified the bHLH transcription factors, ITF-2A and ITF-2B, as the Id-interacting proteins. The levels of expression of these two splice variants did not change during the transition from growing ductal structures to differentiated alveoli. Therefore Id-1 and Id-2, but not the ubiquitous bHLH proteins, appear to represent the key factors whose expression is modulated during different stages of pregnancy in mouse mammary glands.

Original languageEnglish
Pages (from-to)826-830
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume372
Issue number4
DOIs
Publication statusPublished - Aug 8 2008

Fingerprint

Human Mammary Glands
Basic Helix-Loop-Helix Transcription Factors
Genes
Pregnancy
Epithelial Cells
Keratin-8
Keratin-18
Two-Hybrid System Techniques
Proteins
Hybrid systems
Gene expression
Yeast
In Situ Hybridization
Cell Differentiation
Carcinogenesis
Breast
Up-Regulation
Down-Regulation
Cell Culture Techniques
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Expression of Id and ITF-2 genes in the mammary gland during pregnancy. / Itahana, Yoko; Piens, Marie; Fong, Sylvia; Singh, Jarnail; Sumida, Tomoki; Desprez, Pierre Yves.

In: Biochemical and Biophysical Research Communications, Vol. 372, No. 4, 08.08.2008, p. 826-830.

Research output: Contribution to journalArticle

Itahana, Yoko ; Piens, Marie ; Fong, Sylvia ; Singh, Jarnail ; Sumida, Tomoki ; Desprez, Pierre Yves. / Expression of Id and ITF-2 genes in the mammary gland during pregnancy. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 372, No. 4. pp. 826-830.
@article{e6baba3dc1b94bbd8891e58edf5b3d07,
title = "Expression of Id and ITF-2 genes in the mammary gland during pregnancy",
abstract = "Id-1 is one of the four related helix-loop-helix Id proteins that act as inhibitors of the basic helix-loop-helix (bHLH) transcription factors that control cell differentiation, development and carcinogenesis. We previously found that Id-1 regulated the growth, differentiation, apoptosis and invasion of mouse mammary epithelial cells in culture. Using the techniques of immunohistochemistry and in situ hybridization, we now show that Id-1 gene expression is specifically detected in the epithelial cells of growing ductal structures during early pregnancy. Using adjacent sections, we determined that Id-1 was expressed in keratin 8/18 positive cells. We also demonstrated that the up-regulation of Id-2 during late pregnancy correlated with the down-regulation of Id-1. Using the yeast-two hybrid system, we identified the bHLH transcription factors, ITF-2A and ITF-2B, as the Id-interacting proteins. The levels of expression of these two splice variants did not change during the transition from growing ductal structures to differentiated alveoli. Therefore Id-1 and Id-2, but not the ubiquitous bHLH proteins, appear to represent the key factors whose expression is modulated during different stages of pregnancy in mouse mammary glands.",
author = "Yoko Itahana and Marie Piens and Sylvia Fong and Jarnail Singh and Tomoki Sumida and Desprez, {Pierre Yves}",
year = "2008",
month = "8",
day = "8",
doi = "10.1016/j.bbrc.2008.05.139",
language = "English",
volume = "372",
pages = "826--830",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Expression of Id and ITF-2 genes in the mammary gland during pregnancy

AU - Itahana, Yoko

AU - Piens, Marie

AU - Fong, Sylvia

AU - Singh, Jarnail

AU - Sumida, Tomoki

AU - Desprez, Pierre Yves

PY - 2008/8/8

Y1 - 2008/8/8

N2 - Id-1 is one of the four related helix-loop-helix Id proteins that act as inhibitors of the basic helix-loop-helix (bHLH) transcription factors that control cell differentiation, development and carcinogenesis. We previously found that Id-1 regulated the growth, differentiation, apoptosis and invasion of mouse mammary epithelial cells in culture. Using the techniques of immunohistochemistry and in situ hybridization, we now show that Id-1 gene expression is specifically detected in the epithelial cells of growing ductal structures during early pregnancy. Using adjacent sections, we determined that Id-1 was expressed in keratin 8/18 positive cells. We also demonstrated that the up-regulation of Id-2 during late pregnancy correlated with the down-regulation of Id-1. Using the yeast-two hybrid system, we identified the bHLH transcription factors, ITF-2A and ITF-2B, as the Id-interacting proteins. The levels of expression of these two splice variants did not change during the transition from growing ductal structures to differentiated alveoli. Therefore Id-1 and Id-2, but not the ubiquitous bHLH proteins, appear to represent the key factors whose expression is modulated during different stages of pregnancy in mouse mammary glands.

AB - Id-1 is one of the four related helix-loop-helix Id proteins that act as inhibitors of the basic helix-loop-helix (bHLH) transcription factors that control cell differentiation, development and carcinogenesis. We previously found that Id-1 regulated the growth, differentiation, apoptosis and invasion of mouse mammary epithelial cells in culture. Using the techniques of immunohistochemistry and in situ hybridization, we now show that Id-1 gene expression is specifically detected in the epithelial cells of growing ductal structures during early pregnancy. Using adjacent sections, we determined that Id-1 was expressed in keratin 8/18 positive cells. We also demonstrated that the up-regulation of Id-2 during late pregnancy correlated with the down-regulation of Id-1. Using the yeast-two hybrid system, we identified the bHLH transcription factors, ITF-2A and ITF-2B, as the Id-interacting proteins. The levels of expression of these two splice variants did not change during the transition from growing ductal structures to differentiated alveoli. Therefore Id-1 and Id-2, but not the ubiquitous bHLH proteins, appear to represent the key factors whose expression is modulated during different stages of pregnancy in mouse mammary glands.

UR - http://www.scopus.com/inward/record.url?scp=45449092749&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45449092749&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2008.05.139

DO - 10.1016/j.bbrc.2008.05.139

M3 - Article

C2 - 18519032

AN - SCOPUS:45449092749

VL - 372

SP - 826

EP - 830

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -