TY - JOUR
T1 - Expression of inflammation-related genes in aldosterone-producing adenomas with KCNJ5 mutation
AU - Murakami, Masanori
AU - Yoshimoto, Takanobu
AU - Nakano, Yujiro
AU - Tsuchiya, Kyoichiro
AU - Minami, Isao
AU - Bouchi, Ryotaro
AU - Fujii, Yasuhisa
AU - Nakabayashi, Kazuhiko
AU - Hashimoto, Koshi
AU - Hata, Ken ichiro
AU - Kihara, Kazunori
AU - Ogawa, Yoshihiro
N1 - Funding Information:
This study was partially supported by “Advancing Care of Primary Aldosteronism in Japan Study”—Japan Primary Aldosteronism Study (JPAS)—from the Japan Agency for Medical Research and Development, AMED and by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan ( T.Y., 16K08962 and M.M, 16K19392 ).
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/8/5
Y1 - 2016/8/5
N2 - Background The adrenocortical cells have been shown to produce various inflammatory cytokines such as TNFα and IL-6, which could modulate steroidogenesis. However, the role of inflammatory cytokines in aldosterone-producing adenomas (APAs) is not fully understood. In the present study, we examined the relationships between mRNA expression levels of the inflammation-related genes and somatic mutations in APA tissues. Methods We evaluated mRNA expression levels of TNFA, IL6, and NFKB1 in APA tissues obtained from 44 Japanese APA patients. Results We revealed that mRNA expression patterns of the inflammation-related genes depended on a KCNJ5 somatic mutation. In addition, we showed that mRNA expression levels of the inflammation-related genes correlated with those of the steroidogenic enzyme CYP11B1 in the patients with APAs. Conclusion The present study documented for the first time the expression of inflammation-related genes in APAs and the correlation of their expression levels with the KCNJ5 mutation status and mRNA expression levels of steroidogenic enzymes, indicating the pathophysiological relevance of inflammation-related genes in APAs.
AB - Background The adrenocortical cells have been shown to produce various inflammatory cytokines such as TNFα and IL-6, which could modulate steroidogenesis. However, the role of inflammatory cytokines in aldosterone-producing adenomas (APAs) is not fully understood. In the present study, we examined the relationships between mRNA expression levels of the inflammation-related genes and somatic mutations in APA tissues. Methods We evaluated mRNA expression levels of TNFA, IL6, and NFKB1 in APA tissues obtained from 44 Japanese APA patients. Results We revealed that mRNA expression patterns of the inflammation-related genes depended on a KCNJ5 somatic mutation. In addition, we showed that mRNA expression levels of the inflammation-related genes correlated with those of the steroidogenic enzyme CYP11B1 in the patients with APAs. Conclusion The present study documented for the first time the expression of inflammation-related genes in APAs and the correlation of their expression levels with the KCNJ5 mutation status and mRNA expression levels of steroidogenic enzymes, indicating the pathophysiological relevance of inflammation-related genes in APAs.
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U2 - 10.1016/j.bbrc.2016.06.007
DO - 10.1016/j.bbrc.2016.06.007
M3 - Article
C2 - 27282482
AN - SCOPUS:84976626002
VL - 476
SP - 614
EP - 619
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -