Expression of insulin-like growth factor I messenger ribonucleic acid in developing osteophytes in murine experimental osteoarthritis and in rats inoculated with growth hormone-secreting tumor

Ken Okazaki, S. Jingushi, T. Ikenoue, K. Urabe, H. Sakai, A. Ohtsuru, K. Akino, S. Yamashita, S. Nomura, Y. Iwamoto

Research output: Contribution to journalArticle

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Abstract

Osteophytes are one of the characteristic features ofosteoarthritis and are often found in acromegalic arthropathy. The aim of this study was to investigate insulin-like growth factor I (IGF-I) involvement in osteophyte formation. One percent collagenase solution was injected into murine knee joints as an osteoarthritis model. In a different animal group, GH-secreting tumor cells were inoculated sc to the rat thigh as an acromegaly model. A series of osteophyte formation was examined histologically. IGF-I messenger RNA was detected using the in situ hybridization method. Type I IGF receptors were detected immunohistochemically. In the osteoarthritis model, osteophyte formation appeared as synovial or perichondral cell proliferation adjacent to the articular cartilage on day 5, followed by cartilage formation on day 7 and endochondral ossification on day 14. In the acromegaly model, synovial or perichondral cell proliferation was observed 4 weeks after inoculation, followed by osteophyte formation at 8 weeks. In both models, IGF-I messenger RNA and type I IGF receptor were coexpressed by proliferating synovial or perichondral cells, proliferating chondrocytes, and osteoblasts within the developing osteophytes. These results suggest that IGF-I regulated the initiation and development of osteophyte formation in both models in an autocrine and/or paracrine fashion.

Original languageEnglish
Pages (from-to)4821-4830
Number of pages10
JournalEndocrinology
Volume140
Issue number10
DOIs
Publication statusPublished - Jan 1 1999

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Osteophyte
Insulin-Like Growth Factor I
Osteoarthritis
Growth Hormone
RNA
Neoplasms
IGF Type 1 Receptor
Acromegaly
Cell Proliferation
Messenger RNA
Joint Diseases
Articular Cartilage
Collagenases
Chondrocytes
Knee Joint
Thigh
Osteoblasts
Osteogenesis
Cartilage
In Situ Hybridization

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Expression of insulin-like growth factor I messenger ribonucleic acid in developing osteophytes in murine experimental osteoarthritis and in rats inoculated with growth hormone-secreting tumor. / Okazaki, Ken; Jingushi, S.; Ikenoue, T.; Urabe, K.; Sakai, H.; Ohtsuru, A.; Akino, K.; Yamashita, S.; Nomura, S.; Iwamoto, Y.

In: Endocrinology, Vol. 140, No. 10, 01.01.1999, p. 4821-4830.

Research output: Contribution to journalArticle

Okazaki, Ken ; Jingushi, S. ; Ikenoue, T. ; Urabe, K. ; Sakai, H. ; Ohtsuru, A. ; Akino, K. ; Yamashita, S. ; Nomura, S. ; Iwamoto, Y. / Expression of insulin-like growth factor I messenger ribonucleic acid in developing osteophytes in murine experimental osteoarthritis and in rats inoculated with growth hormone-secreting tumor. In: Endocrinology. 1999 ; Vol. 140, No. 10. pp. 4821-4830.
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