Expression of integrin subunits depend on bovine endometrial stromal cells cultured in vitro

Nobuhiko Yamauchi, Keiichiro Kizaki, Osamu Yamada, Toru Takahashi, Chandana B. Herath, Kazuyoshi Hashizume

Research output: Contribution to journalArticle

Abstract

Expression patterns of four integrin a subunits (α2, α3, α5 and αV) and two integrin β subunits (β1 and β3) were examined in bovine endometrial stromal cells (BES) by reverse transcription-polymerase chain reaction and indirect immunofluorescence analysis. All six integrin subunit genes were expressed in the bovine endometrium and in in vitro cultured BES, except α2 subunit gene, which was not expressed with increased generation and longer culture period. The α5 and αV integrin subunit proteins were detected in cultured BES. The α2, α3 and β1 subunit proteins were undetectable in BES derived from pregnant endometrium; however, these subunits are expressed in vivo. These results demonstrate that the expression patterns of integrin subunits are different between endometrium and cultured cells in terms of both mRNA and protein levels. It is indicating that culture conditions influence the expression of integrins in BES.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalConnective Tissue
Volume35
Issue number1
Publication statusPublished - Mar 1 2003
Externally publishedYes

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Stromal Cells
Integrins
Endometrium
Protein Subunits
Indirect Fluorescent Antibody Technique
Genes
Reverse Transcription
In Vitro Techniques
Cultured Cells
Polymerase Chain Reaction
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Rheumatology

Cite this

Yamauchi, N., Kizaki, K., Yamada, O., Takahashi, T., Herath, C. B., & Hashizume, K. (2003). Expression of integrin subunits depend on bovine endometrial stromal cells cultured in vitro. Connective Tissue, 35(1), 1-7.

Expression of integrin subunits depend on bovine endometrial stromal cells cultured in vitro. / Yamauchi, Nobuhiko; Kizaki, Keiichiro; Yamada, Osamu; Takahashi, Toru; Herath, Chandana B.; Hashizume, Kazuyoshi.

In: Connective Tissue, Vol. 35, No. 1, 01.03.2003, p. 1-7.

Research output: Contribution to journalArticle

Yamauchi, N, Kizaki, K, Yamada, O, Takahashi, T, Herath, CB & Hashizume, K 2003, 'Expression of integrin subunits depend on bovine endometrial stromal cells cultured in vitro', Connective Tissue, vol. 35, no. 1, pp. 1-7.
Yamauchi N, Kizaki K, Yamada O, Takahashi T, Herath CB, Hashizume K. Expression of integrin subunits depend on bovine endometrial stromal cells cultured in vitro. Connective Tissue. 2003 Mar 1;35(1):1-7.
Yamauchi, Nobuhiko ; Kizaki, Keiichiro ; Yamada, Osamu ; Takahashi, Toru ; Herath, Chandana B. ; Hashizume, Kazuyoshi. / Expression of integrin subunits depend on bovine endometrial stromal cells cultured in vitro. In: Connective Tissue. 2003 ; Vol. 35, No. 1. pp. 1-7.
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