Expression of MAGE-B genes in esophageal squamous cell carcinoma

Hideki Nagashima, Noriaki Sadanaga, Kohjiro Mashino, Keishi Yamashita, Hiroshi Inoue, Masaki Mori, Keizo Sugimachi

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Abstract

The MAGE-B (MAGE-B1, -B2, -B3, and -B4) genes share strong homology with the MAGE-A gene family. MAGE-B1 and -B2 encode common tumor-specific peptide antigens. There is, however, still very little information about the expression of these genes in human gastro-intestinal carcinomas. We investigated the expression of MAGE-B1 and -B2 genes in 29 cell lines and 53 clinical tumor samples of esophageal squamous cell carcinoma by reverse transcription polymerase chain reaction (RT-PCR). MAGE-B1 and -B2 gene transcripts were detected by RT-PCR in 1 (3%) and 6 (21%) cell lines, and in 9 (17%) and 17 (32%) clinical samples, respectively. Among them, 7/29 (24%) cell lines and 19/53 (36%) clinical samples expressed at least either MAGE-B1 or -B2. A significant correlation was found between negative MAGE-B gene expression and vascular invasion (P=0.008). In 45 out of 53 esophageal carcinoma RNA samples, the MAGE-A1, -A2, and -A3 genes were detected in 27 (60%), 23 (51%), and 30 (67%) samples, respectively, while the MAGE-B genes were detected in 18 (40%) samples. The frequency of MAGE-B gene expression in esophageal carcinoma was relatively higher than that observed for gastric or colorectal carcinomas (12% and 2%, respectively). Therefore, the MAGE-B genes could be used as targets in specific immunotherapy of esophageal squamous cell carcinomas.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalJapanese Journal of Cancer Research
Volume92
Issue number2
DOIs
Publication statusPublished - Jan 1 2001

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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    Nagashima, H., Sadanaga, N., Mashino, K., Yamashita, K., Inoue, H., Mori, M., & Sugimachi, K. (2001). Expression of MAGE-B genes in esophageal squamous cell carcinoma. Japanese Journal of Cancer Research, 92(2), 167-173. https://doi.org/10.1111/j.1349-7006.2001.tb01079.x