Expression of MAGE-B genes in esophageal squamous cell carcinoma

Hideki Nagashima, Noriaki Sadanaga, Kohjiro Mashino, Keishi Yamashita, Hiroshi Inoue, Masaki Mori, Keizo Sugimachi

Research output: Contribution to journalArticle

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Abstract

The MAGE-B (MAGE-B1, -B2, -B3, and -B4) genes share strong homology with the MAGE-A gene family. MAGE-B1 and -B2 encode common tumor-specific peptide antigens. There is, however, still very little information about the expression of these genes in human gastro-intestinal carcinomas. We investigated the expression of MAGE-B1 and -B2 genes in 29 cell lines and 53 clinical tumor samples of esophageal squamous cell carcinoma by reverse transcription polymerase chain reaction (RT-PCR). MAGE-B1 and -B2 gene transcripts were detected by RT-PCR in 1 (3%) and 6 (21%) cell lines, and in 9 (17%) and 17 (32%) clinical samples, respectively. Among them, 7/29 (24%) cell lines and 19/53 (36%) clinical samples expressed at least either MAGE-B1 or -B2. A significant correlation was found between negative MAGE-B gene expression and vascular invasion (P=0.008). In 45 out of 53 esophageal carcinoma RNA samples, the MAGE-A1, -A2, and -A3 genes were detected in 27 (60%), 23 (51%), and 30 (67%) samples, respectively, while the MAGE-B genes were detected in 18 (40%) samples. The frequency of MAGE-B gene expression in esophageal carcinoma was relatively higher than that observed for gastric or colorectal carcinomas (12% and 2%, respectively). Therefore, the MAGE-B genes could be used as targets in specific immunotherapy of esophageal squamous cell carcinomas.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalJapanese Journal of Cancer Research
Volume92
Issue number2
DOIs
Publication statusPublished - Jan 1 2001

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Genes
Carcinoma
Gene Expression
Cell Line
Reverse Transcription
Polymerase Chain Reaction
Esophageal Squamous Cell Carcinoma
varespladib methyl
Immunotherapy
Blood Vessels
Colorectal Neoplasms
Neoplasms
Stomach
RNA
Antigens
Peptides

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Nagashima, H., Sadanaga, N., Mashino, K., Yamashita, K., Inoue, H., Mori, M., & Sugimachi, K. (2001). Expression of MAGE-B genes in esophageal squamous cell carcinoma. Japanese Journal of Cancer Research, 92(2), 167-173. https://doi.org/10.1111/j.1349-7006.2001.tb01079.x

Expression of MAGE-B genes in esophageal squamous cell carcinoma. / Nagashima, Hideki; Sadanaga, Noriaki; Mashino, Kohjiro; Yamashita, Keishi; Inoue, Hiroshi; Mori, Masaki; Sugimachi, Keizo.

In: Japanese Journal of Cancer Research, Vol. 92, No. 2, 01.01.2001, p. 167-173.

Research output: Contribution to journalArticle

Nagashima, H, Sadanaga, N, Mashino, K, Yamashita, K, Inoue, H, Mori, M & Sugimachi, K 2001, 'Expression of MAGE-B genes in esophageal squamous cell carcinoma', Japanese Journal of Cancer Research, vol. 92, no. 2, pp. 167-173. https://doi.org/10.1111/j.1349-7006.2001.tb01079.x
Nagashima, Hideki ; Sadanaga, Noriaki ; Mashino, Kohjiro ; Yamashita, Keishi ; Inoue, Hiroshi ; Mori, Masaki ; Sugimachi, Keizo. / Expression of MAGE-B genes in esophageal squamous cell carcinoma. In: Japanese Journal of Cancer Research. 2001 ; Vol. 92, No. 2. pp. 167-173.
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