Expression of MAGE genes in human colorectal carcinoma

Masaki Mori, Hiroshi Inoue, Koshi Mimori, Kenji Shibuta, Kinya Baba, Hideaki Nakashima, Masaru Haraguchi, Koichi Tsuji, Hiroaki Ueo, Graham F. Barnard, Tsuyoshi Akiyoshi

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Objective: The human genes MAGE-1 and -3 encode tumor-specific peptide antigens, which are recognized by autologous cytotoxic T lymphocytes. The antigens coded by those genes may be useful for cancer immunotherapy. There is, however, little information on the expression of these genes in human colorectal carcinomas. Method: The expression of MAGE-1, -2, and -3 genes in 54 pairs of tumor and corresponding normal tissue specimens of the colorectum was determined by means of reverse transcription polymerase chain reaction. The induction of MAGE-1, -2, -3, and -4 gene expression in eight colorectal carcinoma cell lines also was examined by use of a demethylating agent, 5-Aza- 2'-deoxycytidine (DAC). Results: The expression of MAGE genes was not recognized in normal colorectal tissues at all. In tumor tissue specimens, the expression of MAGE-1, -2, and -3 was recognized in 16 (30%), 15 (28%), and 11 (20%) patients, respectively. The expression was seen frequently in patients with liver metastasis (p < 0.01). Although MAGE-1 or -3 genes were not induced by DAC, MAGE-2 or -4 genes were induced in three of four MAGE-2 negative cell lines or three of seven MAGE-4 negative cell lines, respectively. Conclusions: The MAGE genes were expressed exclusively in tumor tissues of one third of patients with colorectal carcinoma. The identification of such tumor rejection antigens is considered to uncover a new possibility for the specific immunotherapy of colorectal carcinoma. The demethylating agent may increase the number of patients who might be candidates for MAGE-specific immunotherapy.

Original languageEnglish
Pages (from-to)183-188
Number of pages6
JournalAnnals of Surgery
Volume224
Issue number2
DOIs
Publication statusPublished - Aug 27 1996

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Colorectal Neoplasms
Gene Expression
Immunotherapy
Genes
decitabine
Neoplasms
Cell Line
Antigens
Cytotoxic T-Lymphocytes
Neoplasm Antigens
Reverse Transcription
Neoplasm Metastasis
Polymerase Chain Reaction
Peptides
Liver

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Mori, M., Inoue, H., Mimori, K., Shibuta, K., Baba, K., Nakashima, H., ... Akiyoshi, T. (1996). Expression of MAGE genes in human colorectal carcinoma. Annals of Surgery, 224(2), 183-188. https://doi.org/10.1097/00000658-199608000-00011

Expression of MAGE genes in human colorectal carcinoma. / Mori, Masaki; Inoue, Hiroshi; Mimori, Koshi; Shibuta, Kenji; Baba, Kinya; Nakashima, Hideaki; Haraguchi, Masaru; Tsuji, Koichi; Ueo, Hiroaki; Barnard, Graham F.; Akiyoshi, Tsuyoshi.

In: Annals of Surgery, Vol. 224, No. 2, 27.08.1996, p. 183-188.

Research output: Contribution to journalArticle

Mori, M, Inoue, H, Mimori, K, Shibuta, K, Baba, K, Nakashima, H, Haraguchi, M, Tsuji, K, Ueo, H, Barnard, GF & Akiyoshi, T 1996, 'Expression of MAGE genes in human colorectal carcinoma', Annals of Surgery, vol. 224, no. 2, pp. 183-188. https://doi.org/10.1097/00000658-199608000-00011
Mori, Masaki ; Inoue, Hiroshi ; Mimori, Koshi ; Shibuta, Kenji ; Baba, Kinya ; Nakashima, Hideaki ; Haraguchi, Masaru ; Tsuji, Koichi ; Ueo, Hiroaki ; Barnard, Graham F. ; Akiyoshi, Tsuyoshi. / Expression of MAGE genes in human colorectal carcinoma. In: Annals of Surgery. 1996 ; Vol. 224, No. 2. pp. 183-188.
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abstract = "Objective: The human genes MAGE-1 and -3 encode tumor-specific peptide antigens, which are recognized by autologous cytotoxic T lymphocytes. The antigens coded by those genes may be useful for cancer immunotherapy. There is, however, little information on the expression of these genes in human colorectal carcinomas. Method: The expression of MAGE-1, -2, and -3 genes in 54 pairs of tumor and corresponding normal tissue specimens of the colorectum was determined by means of reverse transcription polymerase chain reaction. The induction of MAGE-1, -2, -3, and -4 gene expression in eight colorectal carcinoma cell lines also was examined by use of a demethylating agent, 5-Aza- 2'-deoxycytidine (DAC). Results: The expression of MAGE genes was not recognized in normal colorectal tissues at all. In tumor tissue specimens, the expression of MAGE-1, -2, and -3 was recognized in 16 (30{\%}), 15 (28{\%}), and 11 (20{\%}) patients, respectively. The expression was seen frequently in patients with liver metastasis (p < 0.01). Although MAGE-1 or -3 genes were not induced by DAC, MAGE-2 or -4 genes were induced in three of four MAGE-2 negative cell lines or three of seven MAGE-4 negative cell lines, respectively. Conclusions: The MAGE genes were expressed exclusively in tumor tissues of one third of patients with colorectal carcinoma. The identification of such tumor rejection antigens is considered to uncover a new possibility for the specific immunotherapy of colorectal carcinoma. The demethylating agent may increase the number of patients who might be candidates for MAGE-specific immunotherapy.",
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AU - Inoue, Hiroshi

AU - Mimori, Koshi

AU - Shibuta, Kenji

AU - Baba, Kinya

AU - Nakashima, Hideaki

AU - Haraguchi, Masaru

AU - Tsuji, Koichi

AU - Ueo, Hiroaki

AU - Barnard, Graham F.

AU - Akiyoshi, Tsuyoshi

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N2 - Objective: The human genes MAGE-1 and -3 encode tumor-specific peptide antigens, which are recognized by autologous cytotoxic T lymphocytes. The antigens coded by those genes may be useful for cancer immunotherapy. There is, however, little information on the expression of these genes in human colorectal carcinomas. Method: The expression of MAGE-1, -2, and -3 genes in 54 pairs of tumor and corresponding normal tissue specimens of the colorectum was determined by means of reverse transcription polymerase chain reaction. The induction of MAGE-1, -2, -3, and -4 gene expression in eight colorectal carcinoma cell lines also was examined by use of a demethylating agent, 5-Aza- 2'-deoxycytidine (DAC). Results: The expression of MAGE genes was not recognized in normal colorectal tissues at all. In tumor tissue specimens, the expression of MAGE-1, -2, and -3 was recognized in 16 (30%), 15 (28%), and 11 (20%) patients, respectively. The expression was seen frequently in patients with liver metastasis (p < 0.01). Although MAGE-1 or -3 genes were not induced by DAC, MAGE-2 or -4 genes were induced in three of four MAGE-2 negative cell lines or three of seven MAGE-4 negative cell lines, respectively. Conclusions: The MAGE genes were expressed exclusively in tumor tissues of one third of patients with colorectal carcinoma. The identification of such tumor rejection antigens is considered to uncover a new possibility for the specific immunotherapy of colorectal carcinoma. The demethylating agent may increase the number of patients who might be candidates for MAGE-specific immunotherapy.

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