Expression of MDR1/p-glycoprotein and multidrug resistance-associated protein in childhood solid tumours

Yoshinao Oda, I. Röse, K. Radig, W. Wagemann, U. Mittler, A. Roessner

Research output: Contribution to journalArticle

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Abstract

We evaluated the expression of MDR1/p-glycoprotein in paediatric tumours using reverse transcriptase polymerase chain reaction (RT-PCR), RNA dot blot analysis, and immunohistochemistry on formalin fixed paraffin-embedded material with JSB-1 and C-219 monoclonal antibodies, and compared these three techniques. The expression of multidrug resistance-associated protein (MRP) gene was examined by RT-PCR assay. We studied MDR1/p-glycoprotein and MRP expression in 13 samples from 10 neuroblastoma patients, 11 samples from 10 nephroblastoma patients, 2 rhabdomyosarcomas, 1 adrenocortical carcinoma and 10 benign tumours or tumour-like lesions. Eleven of 13 neuroblastomas, 7 of 11 nephroblastomas, 2 rhabdomyosarcomas, 1 adrenocortical carcinoma, and 7 of 10 benign tumours or tumour-like lesions showed MDR1 PCR products. By RNA dot blot analysis, MDR1 transcripts were detectable in 11 of 34 specimens. Immunohistochemically, we detected positive reaction products for JSB-1 in 26 of 36 samples. There was a significant correlation between the immunoreactivity for JSB-1 and the expression of MDR1 mRNA expression by RT-PCR (P = 0.0001). However, the presence of p-glycoprotein immunostaining does not correlate with the MDR1 expression shown by RT-PCR in every case. As for MRP mRNA expression, 9 of 13 neuroblastomas and 10 of 11 nephroblastomas revealed PCR products.

Original languageEnglish
Pages (from-to)99-105
Number of pages7
JournalVirchows Archiv
Volume430
Issue number2
DOIs
Publication statusPublished - Jan 1 1997

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Multidrug Resistance-Associated Proteins
Glycoproteins
Reverse Transcriptase Polymerase Chain Reaction
Wilms Tumor
Neuroblastoma
Adrenocortical Carcinoma
Neoplasms
RNA
Polymerase Chain Reaction
Messenger RNA
Paraffin
Formaldehyde
Immunohistochemistry
Monoclonal Antibodies
Pediatrics
Genes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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Expression of MDR1/p-glycoprotein and multidrug resistance-associated protein in childhood solid tumours. / Oda, Yoshinao; Röse, I.; Radig, K.; Wagemann, W.; Mittler, U.; Roessner, A.

In: Virchows Archiv, Vol. 430, No. 2, 01.01.1997, p. 99-105.

Research output: Contribution to journalArticle

Oda, Yoshinao ; Röse, I. ; Radig, K. ; Wagemann, W. ; Mittler, U. ; Roessner, A. / Expression of MDR1/p-glycoprotein and multidrug resistance-associated protein in childhood solid tumours. In: Virchows Archiv. 1997 ; Vol. 430, No. 2. pp. 99-105.
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