Expression of mesenchymal markers vimentin and fibronectin: The clinical significance in esophageal squamous cell carcinoma

Tomoya Sudo, Takeshi Iwaya, Naohiro Nishida, Genta Sawada, Yusuke Takahashi, Masahisa Ishibashi, Kohei Shibata, Hiromasa Fujita, Kazuo Shirouzu, Masaki Mori, Koshi Mimori

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background. The importance of mesenchymal characteristics has not been fully elucidated in esophageal cancer. Methods. Ten normal and 77 tumor specimens were collected. Microarray analysis was performed to analyze the expression patterns of epithelial markers, mesenchymal markers, epithelial mesenchymal transition (EMT)-related genes and stem cell markers. RT-PCR analysis was conducted to confirm the results of microarray analysis. Immunohistochemical analysis was performed to verify the level of protein expression. Statistical analysis was performed to investigate the correlation between selected genes and clinicopathological factors. Results. Microarray analysis showed that epithelial markers were significantly down-regulated whereas mesenchymal markers and EMT transcription factors were up-regulated in cancer cells. Two types of gene expression patterns were found in the clustering analysis, type 1 tumors and type 2 tumors. Type 1 tumor clusters did not reveal a fixed gene expression pattern whereas type 2 tumor clusters revealed upregulation of mesenchymal markers EMT inducers and related genes. Vimentin and fibronectin were selected to distinguish between tumor types 1 and 2. Type 2 tumors showed significantly larger tumor sizes (p\0.0001), wider ranges of lymph node metastasis (p = 0.0057), and a more severe clinical stage (p<0.0001) than did type 1 tumors. The prognosis of patients with type 2 tumors was significantly worse than that of patients with type 1 tumors. Univariate and multivariate analyses revealed that classification of type 2 tumors was an independent prognostic factor. Conclusions. The analysis of mesenchymal markers in esophageal cancer is useful in distinguishing patients with a poor prognosis.

Original languageEnglish
Pages (from-to)S324-S335
JournalAnnals of Surgical Oncology
Volume20
Issue number3 SUPPL.
DOIs
Publication statusPublished - Jan 1 2013

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Vimentin
Fibronectins
Neoplasms
Epithelial-Mesenchymal Transition
Microarray Analysis
Esophageal Neoplasms
Esophageal Squamous Cell Carcinoma
Genes
Gene Expression
Cluster Analysis
Transcription Factors
Up-Regulation
Stem Cells
Multivariate Analysis
Lymph Nodes

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

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Expression of mesenchymal markers vimentin and fibronectin : The clinical significance in esophageal squamous cell carcinoma. / Sudo, Tomoya; Iwaya, Takeshi; Nishida, Naohiro; Sawada, Genta; Takahashi, Yusuke; Ishibashi, Masahisa; Shibata, Kohei; Fujita, Hiromasa; Shirouzu, Kazuo; Mori, Masaki; Mimori, Koshi.

In: Annals of Surgical Oncology, Vol. 20, No. 3 SUPPL., 01.01.2013, p. S324-S335.

Research output: Contribution to journalArticle

Sudo, T, Iwaya, T, Nishida, N, Sawada, G, Takahashi, Y, Ishibashi, M, Shibata, K, Fujita, H, Shirouzu, K, Mori, M & Mimori, K 2013, 'Expression of mesenchymal markers vimentin and fibronectin: The clinical significance in esophageal squamous cell carcinoma', Annals of Surgical Oncology, vol. 20, no. 3 SUPPL., pp. S324-S335. https://doi.org/10.1245/s10434-012-2418-z
Sudo, Tomoya ; Iwaya, Takeshi ; Nishida, Naohiro ; Sawada, Genta ; Takahashi, Yusuke ; Ishibashi, Masahisa ; Shibata, Kohei ; Fujita, Hiromasa ; Shirouzu, Kazuo ; Mori, Masaki ; Mimori, Koshi. / Expression of mesenchymal markers vimentin and fibronectin : The clinical significance in esophageal squamous cell carcinoma. In: Annals of Surgical Oncology. 2013 ; Vol. 20, No. 3 SUPPL. pp. S324-S335.
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T2 - The clinical significance in esophageal squamous cell carcinoma

AU - Sudo, Tomoya

AU - Iwaya, Takeshi

AU - Nishida, Naohiro

AU - Sawada, Genta

AU - Takahashi, Yusuke

AU - Ishibashi, Masahisa

AU - Shibata, Kohei

AU - Fujita, Hiromasa

AU - Shirouzu, Kazuo

AU - Mori, Masaki

AU - Mimori, Koshi

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N2 - Background. The importance of mesenchymal characteristics has not been fully elucidated in esophageal cancer. Methods. Ten normal and 77 tumor specimens were collected. Microarray analysis was performed to analyze the expression patterns of epithelial markers, mesenchymal markers, epithelial mesenchymal transition (EMT)-related genes and stem cell markers. RT-PCR analysis was conducted to confirm the results of microarray analysis. Immunohistochemical analysis was performed to verify the level of protein expression. Statistical analysis was performed to investigate the correlation between selected genes and clinicopathological factors. Results. Microarray analysis showed that epithelial markers were significantly down-regulated whereas mesenchymal markers and EMT transcription factors were up-regulated in cancer cells. Two types of gene expression patterns were found in the clustering analysis, type 1 tumors and type 2 tumors. Type 1 tumor clusters did not reveal a fixed gene expression pattern whereas type 2 tumor clusters revealed upregulation of mesenchymal markers EMT inducers and related genes. Vimentin and fibronectin were selected to distinguish between tumor types 1 and 2. Type 2 tumors showed significantly larger tumor sizes (p\0.0001), wider ranges of lymph node metastasis (p = 0.0057), and a more severe clinical stage (p<0.0001) than did type 1 tumors. The prognosis of patients with type 2 tumors was significantly worse than that of patients with type 1 tumors. Univariate and multivariate analyses revealed that classification of type 2 tumors was an independent prognostic factor. Conclusions. The analysis of mesenchymal markers in esophageal cancer is useful in distinguishing patients with a poor prognosis.

AB - Background. The importance of mesenchymal characteristics has not been fully elucidated in esophageal cancer. Methods. Ten normal and 77 tumor specimens were collected. Microarray analysis was performed to analyze the expression patterns of epithelial markers, mesenchymal markers, epithelial mesenchymal transition (EMT)-related genes and stem cell markers. RT-PCR analysis was conducted to confirm the results of microarray analysis. Immunohistochemical analysis was performed to verify the level of protein expression. Statistical analysis was performed to investigate the correlation between selected genes and clinicopathological factors. Results. Microarray analysis showed that epithelial markers were significantly down-regulated whereas mesenchymal markers and EMT transcription factors were up-regulated in cancer cells. Two types of gene expression patterns were found in the clustering analysis, type 1 tumors and type 2 tumors. Type 1 tumor clusters did not reveal a fixed gene expression pattern whereas type 2 tumor clusters revealed upregulation of mesenchymal markers EMT inducers and related genes. Vimentin and fibronectin were selected to distinguish between tumor types 1 and 2. Type 2 tumors showed significantly larger tumor sizes (p\0.0001), wider ranges of lymph node metastasis (p = 0.0057), and a more severe clinical stage (p<0.0001) than did type 1 tumors. The prognosis of patients with type 2 tumors was significantly worse than that of patients with type 1 tumors. Univariate and multivariate analyses revealed that classification of type 2 tumors was an independent prognostic factor. Conclusions. The analysis of mesenchymal markers in esophageal cancer is useful in distinguishing patients with a poor prognosis.

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