Expression of metabotropic glutamate receptor group I in rat gustatory papillae

Takashi Toyono, Yuji Seta, Shinji Kataoka, Shintarou Kawano, Ryuichi Shigemoto, Kuniaki Toyoshima

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Taste-metabotropic glutamate receptor 4 (taste-mGluR4) and the heteromers of T1R1 and T1R3 are candidate receptors involved in the sense of umami (monosodium glutamate) taste. Although the expression of group III mGluRs (taste-mGluR4) has been demonstrated in taste tissues, no mention has been made of the expression of group I mGluRs (mGluR1 and mGluR5) in taste tissues. We examined the expression of mGluR1 and mGluR5 in rat gustatory tissues by using reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization, immunohistochemistry and immunoelectron microscopy. RT-PCR assay showed that mGluR1α and mGluR1β mRNAs were expressed in circumvallate papillae, but mGluR5 mRNA was not expressed. The positive signals of mGluR1 mRNA were detected only in circumvallate taste buds by in situ hybridization analysis. In cryosections of fungiform, foliate and circumvallate papillae, the antibody against mGluRla gave intense labeling on the taste hairs in all taste pores examined. In the developing taste buds, the positive signals of mGluR1α in taste hairs gradually increased with the increase in number of taste bud cells. These results show that, in addition to taste-mGluR4 and the heteromer of T1R1 and T1R3, mGluR1α may function as a receptor for glutamate (umami) taste sensation.

Original languageEnglish
Pages (from-to)29-35
Number of pages7
JournalCell and tissue research
Volume313
Issue number1
DOIs
Publication statusPublished - Jul 1 2003
Externally publishedYes

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Metabotropic Glutamate Receptors
Taste Buds
Hair
Messenger RNA
Reverse Transcription
In Situ Hybridization
Sodium Glutamate
Polymerase Chain Reaction
Immunoelectron Microscopy
Glutamate Receptors
metabotropic glutamate receptor type 1
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Expression of metabotropic glutamate receptor group I in rat gustatory papillae. / Toyono, Takashi; Seta, Yuji; Kataoka, Shinji; Kawano, Shintarou; Shigemoto, Ryuichi; Toyoshima, Kuniaki.

In: Cell and tissue research, Vol. 313, No. 1, 01.07.2003, p. 29-35.

Research output: Contribution to journalArticle

Toyono, Takashi ; Seta, Yuji ; Kataoka, Shinji ; Kawano, Shintarou ; Shigemoto, Ryuichi ; Toyoshima, Kuniaki. / Expression of metabotropic glutamate receptor group I in rat gustatory papillae. In: Cell and tissue research. 2003 ; Vol. 313, No. 1. pp. 29-35.
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