Expression of minichromosome maintenance 5 protein in proliferative and malignant skin diseases

Houjun Liu, Satoshi Takeuchi, Yoichi Moroi, Nengxing Lin, Kazunori Urabe, Hisashi Kokuba, Shinichi Imafuku, Teruki Dainichi, Hiroshi Uchi, Masutaka Furue, Yating Tu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: The entire minichromosome maintenance (MCM) family (MCM2-7) play roles in the initiation and elongation of DNA replication. Many studies have demonstrated that MCM proteins may be better indicators of a wide variety of proliferative or cancer cells in malignant tissues. Objectives: To characterize the pattern and frequency of MCM5 expression in proliferative and malignant skin diseases in comparison with those of proliferating cell nuclear antigen (PCNA). Methods: Twelve normal skin specimens, 12 specimens of psoriasis, 21 specimens of bowenoid papulosis (BP), 16 specimens of Bowen's disease (BD), 38 specimens of skin squamous cell carcinoma (SCC), and 11 specimens of basal cell carcinoma (BCC) were subjected to immunohistochemical staining for MCM5 and PCNA. Results: MCM5 protein was expressed in the lower layers of epidermis in psoriasis, while MCM5 protein were present throughout the tumor cells in BP, BD, and moderately/poorly differentiated SCC. MCM5 protein was preferentially expressed in the periphery of well-differentiated SCC or bigger nests of BCC, although some small nests of BCC seemingly showed diffuse staining patterns. The percentages of MCM5-positive cells were 15.7% in normal skin, 21.8% in psoriasis, 75.9% in BP, 83.8% in BD, 63.5% in well-differentiated SCC, 77.5% in moderately differentiated SCC, 79.8% in poorly differentiated SCC, and 21.2% in BCC in average. Well-differentiated SCC showed a significantly lower percentage of positive cells than did moderately differentiated SCC or poorly differentiated SCC. MCM5 staining basically show a similar staining pattern to that of PCNA, but more cells tended to be stained with MCM5 than with PCNA. Conclusions: Our results demonstrate pattern and frequency of MCM5 expression in various skin diseases and suggest that MCM5 may be a useful marker to detect cell proliferation in skin tissue sections.

Original languageEnglish
Pages (from-to)1171-1176
Number of pages6
JournalInternational Journal of Dermatology
Volume46
Issue number11
DOIs
Publication statusPublished - Nov 1 2007

Fingerprint

Minichromosome Maintenance Proteins
Skin Diseases
Squamous Cell Carcinoma
Basal Cell Carcinoma
Proliferating Cell Nuclear Antigen
Bowen's Disease
Psoriasis
Staining and Labeling
Skin
Proteins
DNA Replication
Epidermis
Neoplasms
Maintenance
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Expression of minichromosome maintenance 5 protein in proliferative and malignant skin diseases. / Liu, Houjun; Takeuchi, Satoshi; Moroi, Yoichi; Lin, Nengxing; Urabe, Kazunori; Kokuba, Hisashi; Imafuku, Shinichi; Dainichi, Teruki; Uchi, Hiroshi; Furue, Masutaka; Tu, Yating.

In: International Journal of Dermatology, Vol. 46, No. 11, 01.11.2007, p. 1171-1176.

Research output: Contribution to journalArticle

Liu, H, Takeuchi, S, Moroi, Y, Lin, N, Urabe, K, Kokuba, H, Imafuku, S, Dainichi, T, Uchi, H, Furue, M & Tu, Y 2007, 'Expression of minichromosome maintenance 5 protein in proliferative and malignant skin diseases', International Journal of Dermatology, vol. 46, no. 11, pp. 1171-1176. https://doi.org/10.1111/j.1365-4632.2007.03335.x
Liu, Houjun ; Takeuchi, Satoshi ; Moroi, Yoichi ; Lin, Nengxing ; Urabe, Kazunori ; Kokuba, Hisashi ; Imafuku, Shinichi ; Dainichi, Teruki ; Uchi, Hiroshi ; Furue, Masutaka ; Tu, Yating. / Expression of minichromosome maintenance 5 protein in proliferative and malignant skin diseases. In: International Journal of Dermatology. 2007 ; Vol. 46, No. 11. pp. 1171-1176.
@article{3b341f16ffd247da92c883522e9f6d8e,
title = "Expression of minichromosome maintenance 5 protein in proliferative and malignant skin diseases",
abstract = "Background: The entire minichromosome maintenance (MCM) family (MCM2-7) play roles in the initiation and elongation of DNA replication. Many studies have demonstrated that MCM proteins may be better indicators of a wide variety of proliferative or cancer cells in malignant tissues. Objectives: To characterize the pattern and frequency of MCM5 expression in proliferative and malignant skin diseases in comparison with those of proliferating cell nuclear antigen (PCNA). Methods: Twelve normal skin specimens, 12 specimens of psoriasis, 21 specimens of bowenoid papulosis (BP), 16 specimens of Bowen's disease (BD), 38 specimens of skin squamous cell carcinoma (SCC), and 11 specimens of basal cell carcinoma (BCC) were subjected to immunohistochemical staining for MCM5 and PCNA. Results: MCM5 protein was expressed in the lower layers of epidermis in psoriasis, while MCM5 protein were present throughout the tumor cells in BP, BD, and moderately/poorly differentiated SCC. MCM5 protein was preferentially expressed in the periphery of well-differentiated SCC or bigger nests of BCC, although some small nests of BCC seemingly showed diffuse staining patterns. The percentages of MCM5-positive cells were 15.7{\%} in normal skin, 21.8{\%} in psoriasis, 75.9{\%} in BP, 83.8{\%} in BD, 63.5{\%} in well-differentiated SCC, 77.5{\%} in moderately differentiated SCC, 79.8{\%} in poorly differentiated SCC, and 21.2{\%} in BCC in average. Well-differentiated SCC showed a significantly lower percentage of positive cells than did moderately differentiated SCC or poorly differentiated SCC. MCM5 staining basically show a similar staining pattern to that of PCNA, but more cells tended to be stained with MCM5 than with PCNA. Conclusions: Our results demonstrate pattern and frequency of MCM5 expression in various skin diseases and suggest that MCM5 may be a useful marker to detect cell proliferation in skin tissue sections.",
author = "Houjun Liu and Satoshi Takeuchi and Yoichi Moroi and Nengxing Lin and Kazunori Urabe and Hisashi Kokuba and Shinichi Imafuku and Teruki Dainichi and Hiroshi Uchi and Masutaka Furue and Yating Tu",
year = "2007",
month = "11",
day = "1",
doi = "10.1111/j.1365-4632.2007.03335.x",
language = "English",
volume = "46",
pages = "1171--1176",
journal = "International Journal of Dermatology",
issn = "0011-9059",
publisher = "Wiley-Blackwell",
number = "11",

}

TY - JOUR

T1 - Expression of minichromosome maintenance 5 protein in proliferative and malignant skin diseases

AU - Liu, Houjun

AU - Takeuchi, Satoshi

AU - Moroi, Yoichi

AU - Lin, Nengxing

AU - Urabe, Kazunori

AU - Kokuba, Hisashi

AU - Imafuku, Shinichi

AU - Dainichi, Teruki

AU - Uchi, Hiroshi

AU - Furue, Masutaka

AU - Tu, Yating

PY - 2007/11/1

Y1 - 2007/11/1

N2 - Background: The entire minichromosome maintenance (MCM) family (MCM2-7) play roles in the initiation and elongation of DNA replication. Many studies have demonstrated that MCM proteins may be better indicators of a wide variety of proliferative or cancer cells in malignant tissues. Objectives: To characterize the pattern and frequency of MCM5 expression in proliferative and malignant skin diseases in comparison with those of proliferating cell nuclear antigen (PCNA). Methods: Twelve normal skin specimens, 12 specimens of psoriasis, 21 specimens of bowenoid papulosis (BP), 16 specimens of Bowen's disease (BD), 38 specimens of skin squamous cell carcinoma (SCC), and 11 specimens of basal cell carcinoma (BCC) were subjected to immunohistochemical staining for MCM5 and PCNA. Results: MCM5 protein was expressed in the lower layers of epidermis in psoriasis, while MCM5 protein were present throughout the tumor cells in BP, BD, and moderately/poorly differentiated SCC. MCM5 protein was preferentially expressed in the periphery of well-differentiated SCC or bigger nests of BCC, although some small nests of BCC seemingly showed diffuse staining patterns. The percentages of MCM5-positive cells were 15.7% in normal skin, 21.8% in psoriasis, 75.9% in BP, 83.8% in BD, 63.5% in well-differentiated SCC, 77.5% in moderately differentiated SCC, 79.8% in poorly differentiated SCC, and 21.2% in BCC in average. Well-differentiated SCC showed a significantly lower percentage of positive cells than did moderately differentiated SCC or poorly differentiated SCC. MCM5 staining basically show a similar staining pattern to that of PCNA, but more cells tended to be stained with MCM5 than with PCNA. Conclusions: Our results demonstrate pattern and frequency of MCM5 expression in various skin diseases and suggest that MCM5 may be a useful marker to detect cell proliferation in skin tissue sections.

AB - Background: The entire minichromosome maintenance (MCM) family (MCM2-7) play roles in the initiation and elongation of DNA replication. Many studies have demonstrated that MCM proteins may be better indicators of a wide variety of proliferative or cancer cells in malignant tissues. Objectives: To characterize the pattern and frequency of MCM5 expression in proliferative and malignant skin diseases in comparison with those of proliferating cell nuclear antigen (PCNA). Methods: Twelve normal skin specimens, 12 specimens of psoriasis, 21 specimens of bowenoid papulosis (BP), 16 specimens of Bowen's disease (BD), 38 specimens of skin squamous cell carcinoma (SCC), and 11 specimens of basal cell carcinoma (BCC) were subjected to immunohistochemical staining for MCM5 and PCNA. Results: MCM5 protein was expressed in the lower layers of epidermis in psoriasis, while MCM5 protein were present throughout the tumor cells in BP, BD, and moderately/poorly differentiated SCC. MCM5 protein was preferentially expressed in the periphery of well-differentiated SCC or bigger nests of BCC, although some small nests of BCC seemingly showed diffuse staining patterns. The percentages of MCM5-positive cells were 15.7% in normal skin, 21.8% in psoriasis, 75.9% in BP, 83.8% in BD, 63.5% in well-differentiated SCC, 77.5% in moderately differentiated SCC, 79.8% in poorly differentiated SCC, and 21.2% in BCC in average. Well-differentiated SCC showed a significantly lower percentage of positive cells than did moderately differentiated SCC or poorly differentiated SCC. MCM5 staining basically show a similar staining pattern to that of PCNA, but more cells tended to be stained with MCM5 than with PCNA. Conclusions: Our results demonstrate pattern and frequency of MCM5 expression in various skin diseases and suggest that MCM5 may be a useful marker to detect cell proliferation in skin tissue sections.

UR - http://www.scopus.com/inward/record.url?scp=35848956356&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35848956356&partnerID=8YFLogxK

U2 - 10.1111/j.1365-4632.2007.03335.x

DO - 10.1111/j.1365-4632.2007.03335.x

M3 - Article

C2 - 17988337

AN - SCOPUS:35848956356

VL - 46

SP - 1171

EP - 1176

JO - International Journal of Dermatology

JF - International Journal of Dermatology

SN - 0011-9059

IS - 11

ER -