Expression of mouse mammary tumor virus superantigen accelerates tumorigenicity of myeloma cells

M. Umemura, W. Wajjwalku, N. Upragarin, T. Liu, H. Nishimura, T. Matsuguchi, Y. Nishiyama, G. M. Wilson, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    To investigate whether superantigen (SAG) from endogenous mouse mammary tumor virus functions as an immunogenic or a tumorigenic factor in tumor development, the BALB/c myeloma cell line FO was transfected with the SAG gene from the 3' Mtv-50 long terminal repeat (LTR) open reading frame (ORF), the product of which was specific for Vβ6. All five transfectants expressing Mtv-50 LTR ORF mRNA showed stimulatory activity for Vβ6 T-cell hybridomas in vitro; this activity was inhibited by the addition of anti-Mtv-7 monoclonal antibody (MAb) or anti-major histocompatibility complex class II I-A(d) and I-E(d) MAb. All transfectants with the SAG gene grew more rapidly than did mock transfectants in BALB/c mice after subcutaneous inoculation, whereas all clones, including mock transfectants, grew equally well in athymic nude mice. A significant fraction of Vβ6 T cells selectively expressed activation markers, including CD44(high), CD62L(low), and CD69(high), and produced large amounts of interleukin 5 (IL-5) and IL-6 in BALB/c mice inoculated with transfectants. These results suggested that the expression of viral SAG enhances the tumorigenicity of a myeloma cell line through the stimulation of SAG-reactive T cells.

    Original languageEnglish
    Pages (from-to)8226-8233
    Number of pages8
    JournalJournal of Virology
    Volume74
    Issue number18
    DOIs
    Publication statusPublished - Sep 13 2000

    Fingerprint

    Mouse mammary tumor virus
    superantigens
    Superantigens
    myeloma
    terminal repeat sequences
    Terminal Repeat Sequences
    T-lymphocytes
    T-Lymphocytes
    Nude Mice
    Open Reading Frames
    cells
    open reading frames
    monoclonal antibodies
    mice
    Monoclonal Antibodies
    cell lines
    Cell Line
    interleukin-5
    hybridomas
    Interleukin-5

    All Science Journal Classification (ASJC) codes

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

    Cite this

    Umemura, M., Wajjwalku, W., Upragarin, N., Liu, T., Nishimura, H., Matsuguchi, T., ... Yoshikai, Y. (2000). Expression of mouse mammary tumor virus superantigen accelerates tumorigenicity of myeloma cells. Journal of Virology, 74(18), 8226-8233. https://doi.org/10.1128/JVI.74.18.8226-8233.2000

    Expression of mouse mammary tumor virus superantigen accelerates tumorigenicity of myeloma cells. / Umemura, M.; Wajjwalku, W.; Upragarin, N.; Liu, T.; Nishimura, H.; Matsuguchi, T.; Nishiyama, Y.; Wilson, G. M.; Yoshikai, Yasunobu.

    In: Journal of Virology, Vol. 74, No. 18, 13.09.2000, p. 8226-8233.

    Research output: Contribution to journalArticle

    Umemura, M, Wajjwalku, W, Upragarin, N, Liu, T, Nishimura, H, Matsuguchi, T, Nishiyama, Y, Wilson, GM & Yoshikai, Y 2000, 'Expression of mouse mammary tumor virus superantigen accelerates tumorigenicity of myeloma cells', Journal of Virology, vol. 74, no. 18, pp. 8226-8233. https://doi.org/10.1128/JVI.74.18.8226-8233.2000
    Umemura M, Wajjwalku W, Upragarin N, Liu T, Nishimura H, Matsuguchi T et al. Expression of mouse mammary tumor virus superantigen accelerates tumorigenicity of myeloma cells. Journal of Virology. 2000 Sep 13;74(18):8226-8233. https://doi.org/10.1128/JVI.74.18.8226-8233.2000
    Umemura, M. ; Wajjwalku, W. ; Upragarin, N. ; Liu, T. ; Nishimura, H. ; Matsuguchi, T. ; Nishiyama, Y. ; Wilson, G. M. ; Yoshikai, Yasunobu. / Expression of mouse mammary tumor virus superantigen accelerates tumorigenicity of myeloma cells. In: Journal of Virology. 2000 ; Vol. 74, No. 18. pp. 8226-8233.
    @article{24195d4a07dd489ea345f038d0a2cc2b,
    title = "Expression of mouse mammary tumor virus superantigen accelerates tumorigenicity of myeloma cells",
    abstract = "To investigate whether superantigen (SAG) from endogenous mouse mammary tumor virus functions as an immunogenic or a tumorigenic factor in tumor development, the BALB/c myeloma cell line FO was transfected with the SAG gene from the 3' Mtv-50 long terminal repeat (LTR) open reading frame (ORF), the product of which was specific for Vβ6. All five transfectants expressing Mtv-50 LTR ORF mRNA showed stimulatory activity for Vβ6 T-cell hybridomas in vitro; this activity was inhibited by the addition of anti-Mtv-7 monoclonal antibody (MAb) or anti-major histocompatibility complex class II I-A(d) and I-E(d) MAb. All transfectants with the SAG gene grew more rapidly than did mock transfectants in BALB/c mice after subcutaneous inoculation, whereas all clones, including mock transfectants, grew equally well in athymic nude mice. A significant fraction of Vβ6 T cells selectively expressed activation markers, including CD44(high), CD62L(low), and CD69(high), and produced large amounts of interleukin 5 (IL-5) and IL-6 in BALB/c mice inoculated with transfectants. These results suggested that the expression of viral SAG enhances the tumorigenicity of a myeloma cell line through the stimulation of SAG-reactive T cells.",
    author = "M. Umemura and W. Wajjwalku and N. Upragarin and T. Liu and H. Nishimura and T. Matsuguchi and Y. Nishiyama and Wilson, {G. M.} and Yasunobu Yoshikai",
    year = "2000",
    month = "9",
    day = "13",
    doi = "10.1128/JVI.74.18.8226-8233.2000",
    language = "English",
    volume = "74",
    pages = "8226--8233",
    journal = "Journal of Virology",
    issn = "0022-538X",
    publisher = "American Society for Microbiology",
    number = "18",

    }

    TY - JOUR

    T1 - Expression of mouse mammary tumor virus superantigen accelerates tumorigenicity of myeloma cells

    AU - Umemura, M.

    AU - Wajjwalku, W.

    AU - Upragarin, N.

    AU - Liu, T.

    AU - Nishimura, H.

    AU - Matsuguchi, T.

    AU - Nishiyama, Y.

    AU - Wilson, G. M.

    AU - Yoshikai, Yasunobu

    PY - 2000/9/13

    Y1 - 2000/9/13

    N2 - To investigate whether superantigen (SAG) from endogenous mouse mammary tumor virus functions as an immunogenic or a tumorigenic factor in tumor development, the BALB/c myeloma cell line FO was transfected with the SAG gene from the 3' Mtv-50 long terminal repeat (LTR) open reading frame (ORF), the product of which was specific for Vβ6. All five transfectants expressing Mtv-50 LTR ORF mRNA showed stimulatory activity for Vβ6 T-cell hybridomas in vitro; this activity was inhibited by the addition of anti-Mtv-7 monoclonal antibody (MAb) or anti-major histocompatibility complex class II I-A(d) and I-E(d) MAb. All transfectants with the SAG gene grew more rapidly than did mock transfectants in BALB/c mice after subcutaneous inoculation, whereas all clones, including mock transfectants, grew equally well in athymic nude mice. A significant fraction of Vβ6 T cells selectively expressed activation markers, including CD44(high), CD62L(low), and CD69(high), and produced large amounts of interleukin 5 (IL-5) and IL-6 in BALB/c mice inoculated with transfectants. These results suggested that the expression of viral SAG enhances the tumorigenicity of a myeloma cell line through the stimulation of SAG-reactive T cells.

    AB - To investigate whether superantigen (SAG) from endogenous mouse mammary tumor virus functions as an immunogenic or a tumorigenic factor in tumor development, the BALB/c myeloma cell line FO was transfected with the SAG gene from the 3' Mtv-50 long terminal repeat (LTR) open reading frame (ORF), the product of which was specific for Vβ6. All five transfectants expressing Mtv-50 LTR ORF mRNA showed stimulatory activity for Vβ6 T-cell hybridomas in vitro; this activity was inhibited by the addition of anti-Mtv-7 monoclonal antibody (MAb) or anti-major histocompatibility complex class II I-A(d) and I-E(d) MAb. All transfectants with the SAG gene grew more rapidly than did mock transfectants in BALB/c mice after subcutaneous inoculation, whereas all clones, including mock transfectants, grew equally well in athymic nude mice. A significant fraction of Vβ6 T cells selectively expressed activation markers, including CD44(high), CD62L(low), and CD69(high), and produced large amounts of interleukin 5 (IL-5) and IL-6 in BALB/c mice inoculated with transfectants. These results suggested that the expression of viral SAG enhances the tumorigenicity of a myeloma cell line through the stimulation of SAG-reactive T cells.

    UR - http://www.scopus.com/inward/record.url?scp=0033863968&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0033863968&partnerID=8YFLogxK

    U2 - 10.1128/JVI.74.18.8226-8233.2000

    DO - 10.1128/JVI.74.18.8226-8233.2000

    M3 - Article

    VL - 74

    SP - 8226

    EP - 8233

    JO - Journal of Virology

    JF - Journal of Virology

    SN - 0022-538X

    IS - 18

    ER -