Expression of mRNA in the frontal cortex and hypothalamus in a rat model of acute carbon dioxide poisoning

Kazuo Sato, Akiko Tsuji, Yosuke Usumoto, Keiko Kudo, Takeshi Yokoyama, Noriaki Ikeda

Research output: Contribution to journalArticle

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Abstract

Acute carbon dioxide (CO2) poisoning causes no specific features that are revealed upon autopsy, and the pathophysiological mechanism of this syndrome is unclear. To address this issue, in the present study, we exposed rats to CO2 concentrations ranging from 10% to 60% and determined the effects on mRNA expression. According to the results of Gene Ontology (GO) and cluster analyses of microarrays data, we selected the following genes for further analysis: alkylglycerone phosphate synthase (Agps), hypocretin (Hcrt), tyrosine hydroxylase (Th), heat shock protein beta 2 (Hspb2), and opioid receptor delta 1 (Oprd1) expressed in the frontal cortex and renin (Ren), pancreatic polypeptide (Ppy), corticotropin releasing hormone receptor 2 (Crhr2), carbonic anhydrase 1 (Car1), and hypocretin receptor 1 (Hcrtr1) expressed in the hypothalamus. We found significant differences between the expression levels of Agps and Hspb2 mRNAs in the frontal cortex and that of Ppy, Crhr2 mRNAs in the hypothalamus in the presence of high concentrations of CO2. Further investigation of these genes may clarify the pathophysiology of acute CO2 poisoning and facilitate the development of novel forensic tests that can diagnose the cause of death.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalLegal Medicine
Volume19
DOIs
Publication statusPublished - Mar 1 2016

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Frontal Lobe
Orexin Receptors
Carbon Dioxide
Poisoning
Hypothalamus
Corticotropin-Releasing Hormone Receptors
Pancreatic Polypeptide
Heat-Shock Proteins
Messenger RNA
delta Opioid Receptor
Gene Ontology
Carbonic Anhydrases
Tyrosine 3-Monooxygenase
Multigene Family
Renin
Genes
Cluster Analysis
Cause of Death
Autopsy
alkylglycerone-phosphate synthase

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Issues, ethics and legal aspects

Cite this

Expression of mRNA in the frontal cortex and hypothalamus in a rat model of acute carbon dioxide poisoning. / Sato, Kazuo; Tsuji, Akiko; Usumoto, Yosuke; Kudo, Keiko; Yokoyama, Takeshi; Ikeda, Noriaki.

In: Legal Medicine, Vol. 19, 01.03.2016, p. 101-106.

Research output: Contribution to journalArticle

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