Expression of NG2 proteoglycan during endochondral and intramembranous ossification

Jun-Ichi Fukushi, Masaru Inatani, Yu Yamaguchi, William B. Stallcup

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We have used immunohistochemistry to study the distribution of the NG2 proteoglycan during bone development in the mouse. At embryonic day 15.5, NG2 was strongly detected in the immature cartilage of developing limbs. After transient down-regulation in mature chondrocytes, NG2 was up-regulated during primary ossification, colocalizing with alkaline phosphatase and tenascin C. In the epiphyseal growth plates of newborn mouse tibia, NG2 and alkaline phosphatase exhibited overlapping patterns of expression by hypertrophic chondrocytes and by osteoblasts surrounding newly formed bone trabeculae. NG2 was down-regulated after puberty, being only faintly detectable in the tibial growth plates of 3-month-old mice. In cranial sutures, NG2 was strongly labeled in osteogenic bone fronts and in the suture matrix. Our results indicate that NG2 expression is up-regulated during both endochondral and intramembranous ossification, but is down-regulated as ossification is completed.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalDevelopmental Dynamics
Volume228
Issue number1
DOIs
Publication statusPublished - Sep 1 2003

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Growth Plate
Osteogenesis
Chondrocytes
Alkaline Phosphatase
Cranial Sutures
Tenascin
Bone and Bones
Bone Development
Puberty
Osteoblasts
Tibia
Sutures
Cartilage
Down-Regulation
Extremities
Immunohistochemistry
chondroitin sulfate proteoglycan 4

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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Expression of NG2 proteoglycan during endochondral and intramembranous ossification. / Fukushi, Jun-Ichi; Inatani, Masaru; Yamaguchi, Yu; Stallcup, William B.

In: Developmental Dynamics, Vol. 228, No. 1, 01.09.2003, p. 143-148.

Research output: Contribution to journalArticle

Fukushi, J-I, Inatani, M, Yamaguchi, Y & Stallcup, WB 2003, 'Expression of NG2 proteoglycan during endochondral and intramembranous ossification', Developmental Dynamics, vol. 228, no. 1, pp. 143-148. https://doi.org/10.1002/dvdy.10359
Fukushi, Jun-Ichi ; Inatani, Masaru ; Yamaguchi, Yu ; Stallcup, William B. / Expression of NG2 proteoglycan during endochondral and intramembranous ossification. In: Developmental Dynamics. 2003 ; Vol. 228, No. 1. pp. 143-148.
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