Expression of NG2 proteoglycan during endochondral and intramembranous ossification

Jun Ichi Fukushi, Masaru Inatani, Yu Yamaguchi, William B. Stallcup

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


We have used immunohistochemistry to study the distribution of the NG2 proteoglycan during bone development in the mouse. At embryonic day 15.5, NG2 was strongly detected in the immature cartilage of developing limbs. After transient down-regulation in mature chondrocytes, NG2 was up-regulated during primary ossification, colocalizing with alkaline phosphatase and tenascin C. In the epiphyseal growth plates of newborn mouse tibia, NG2 and alkaline phosphatase exhibited overlapping patterns of expression by hypertrophic chondrocytes and by osteoblasts surrounding newly formed bone trabeculae. NG2 was down-regulated after puberty, being only faintly detectable in the tibial growth plates of 3-month-old mice. In cranial sutures, NG2 was strongly labeled in osteogenic bone fronts and in the suture matrix. Our results indicate that NG2 expression is up-regulated during both endochondral and intramembranous ossification, but is down-regulated as ossification is completed.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalDevelopmental Dynamics
Issue number1
Publication statusPublished - Sept 1 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Developmental Biology


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