Expression of osteocalcin and Jun D in the early period during reactionary dentin formation after tooth preparation in rat molars

Masako Hirata, Takayoshi Yamaza, Yu Feng Mei, Akifumi Akamine

Research output: Contribution to journalArticle

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Abstract

We examined, in rats, the expression of osteocalcin and Jun D in the early stage of reactionary dentin formation after tooth preparation and the accompanying morphological changes. Reverse transcription/polymerase chain reaction analysis revealed strong expression of osteocalcin mRNA in pulp tissue at 2 and 3 days post-preparation compared with that in control teeth. Light microscopy demonstrated that, at the dentin-pulp interface, damaged odontoblasts were detached from the dentin matrix immediately after preparation, with neutrophils lining the dental surface after 1 day. After 2-3 days, differentiated odontoblasts appeared at the interface. Reactionary dentin with tubular structures was formed under the cavity after 10 days. Immunoelectron microscopy showed that trace amounts of osteocalcin were expressed in odontoblasts at 2 days post-preparation, and abundant osteocalcin was found in the highly developed Golgi apparatus and granules at 3 days post-preparation. Osteocalcin was also found on type I collagen fibrils in newly formed predentin. The existing dentinal tubules were filled with osteocalcin-coated type I collagen fibrils. We observed, by immnohistochemistry, that Jun D was temporally expressed in the nuclei of the odontoblasts at 1 and 2 days post-preparation. However, no Jun D was found in the dental pulp cells at any other time or in control teeth. Thus, osteocalcin expression is correlated with reactionary dentin formation, and Jun D is associated with osteocalcin expression in odontoblasts. Osteocalcin may also serve as an obturator of the dentinal tubules to protect dental pulp vitality against external irritants after preparation.

Original languageEnglish
Pages (from-to)455-465
Number of pages11
JournalCell and tissue research
Volume319
Issue number3
DOIs
Publication statusPublished - Mar 1 2005

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Tooth Preparation
Osteocalcin
Dentin
Rats
Odontoblasts
Pulp
Dental Pulp
Tooth
Collagen Type I
Immunoelectron Microscopy
Irritants
Polymerase chain reaction
Golgi Apparatus
Transcription
Linings
Reverse Transcription
Optical microscopy
Microscopy
Microscopic examination
Neutrophils

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Expression of osteocalcin and Jun D in the early period during reactionary dentin formation after tooth preparation in rat molars. / Hirata, Masako; Yamaza, Takayoshi; Mei, Yu Feng; Akamine, Akifumi.

In: Cell and tissue research, Vol. 319, No. 3, 01.03.2005, p. 455-465.

Research output: Contribution to journalArticle

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