Expression of phosphorylated Stat3, cyclin D1 and Bcl-xL in extramammary Paget disease

H. J. Liu, Y. Moroi, T. Masuda, S. Yasumoto, H. Kokuba, S. Imafuku, T. Koga, T. Tetsuya, Y. T. Tu, H. Aburatani, Masutaka Furue, K. Urabe

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Abstract

Background: Stat3 (Signal transducer and activator of transcription-3) is an oncogene that plays a critical role in regulating fundamental processes associated with malignant transformation and cell survival. It participates in oncogenesis through upregulation of genes encoding apoptosis inhibitors (Bcl-xL) and cell cycle regulators (cyclin D1). The expression of Stat3, Bcl-xL and cyclin D1 protein has not been investigated in extramammary Paget disease (EMPD). Objectives: To study the expression of phosphorylated Stat3 (p-Stat3), Bcl-xL and cyclin D1 protein in EMPD and to evaluate the relationships among them. Methods: Thirty-six tissue samples from 34 patients with primary EMPD were subjected to immunohistochemical staining for p-Stat3, cyclin D1 and Bcl-xL. Results: Thirty-five of 36 specimens were clearly positive for p-Stat3 in EMPD, while 30 of 36 and 32 of 36 were positive for cyclin D1 and Bcl-xL expression, respectively. In all of four invasive EMPD specimens, strong and frequent expression of these three molecules was evident; moreover, two invasive EMPD specimens with lymph nodal metastasis showed very strong nuclear and membranous p-Stat3 staining. Two metastatic lymph node specimens showed very strong nuclear and local membrane p-Stat3 staining. There were significant correlations between p-Stat3 and cyclin D1 expression and between p-Stat3 and Bcl-xL expression. Conclusions: Our study shows that the expression of p-Stat3, cyclin D 1 and Bcl-xL may play a pivotal role in the pathogenesis of EMPD.

Original languageEnglish
Pages (from-to)926-932
Number of pages7
JournalBritish Journal of Dermatology
Volume154
Issue number5
DOIs
Publication statusPublished - May 1 2006

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Extramammary Paget's Disease
STAT3 Transcription Factor
Cyclin D1
Staining and Labeling
Cyclin D
Nuclear Envelope
Lymph
Oncogenes
Cell Survival
Cell Cycle
Carcinogenesis
Proteins
Up-Regulation
Lymph Nodes
Apoptosis
Neoplasm Metastasis

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Liu, H. J., Moroi, Y., Masuda, T., Yasumoto, S., Kokuba, H., Imafuku, S., ... Urabe, K. (2006). Expression of phosphorylated Stat3, cyclin D1 and Bcl-xL in extramammary Paget disease. British Journal of Dermatology, 154(5), 926-932. https://doi.org/10.1111/j.1365-2133.2005.06951.x

Expression of phosphorylated Stat3, cyclin D1 and Bcl-xL in extramammary Paget disease. / Liu, H. J.; Moroi, Y.; Masuda, T.; Yasumoto, S.; Kokuba, H.; Imafuku, S.; Koga, T.; Tetsuya, T.; Tu, Y. T.; Aburatani, H.; Furue, Masutaka; Urabe, K.

In: British Journal of Dermatology, Vol. 154, No. 5, 01.05.2006, p. 926-932.

Research output: Contribution to journalArticle

Liu, HJ, Moroi, Y, Masuda, T, Yasumoto, S, Kokuba, H, Imafuku, S, Koga, T, Tetsuya, T, Tu, YT, Aburatani, H, Furue, M & Urabe, K 2006, 'Expression of phosphorylated Stat3, cyclin D1 and Bcl-xL in extramammary Paget disease', British Journal of Dermatology, vol. 154, no. 5, pp. 926-932. https://doi.org/10.1111/j.1365-2133.2005.06951.x
Liu, H. J. ; Moroi, Y. ; Masuda, T. ; Yasumoto, S. ; Kokuba, H. ; Imafuku, S. ; Koga, T. ; Tetsuya, T. ; Tu, Y. T. ; Aburatani, H. ; Furue, Masutaka ; Urabe, K. / Expression of phosphorylated Stat3, cyclin D1 and Bcl-xL in extramammary Paget disease. In: British Journal of Dermatology. 2006 ; Vol. 154, No. 5. pp. 926-932.
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T1 - Expression of phosphorylated Stat3, cyclin D1 and Bcl-xL in extramammary Paget disease

AU - Liu, H. J.

AU - Moroi, Y.

AU - Masuda, T.

AU - Yasumoto, S.

AU - Kokuba, H.

AU - Imafuku, S.

AU - Koga, T.

AU - Tetsuya, T.

AU - Tu, Y. T.

AU - Aburatani, H.

AU - Furue, Masutaka

AU - Urabe, K.

PY - 2006/5/1

Y1 - 2006/5/1

N2 - Background: Stat3 (Signal transducer and activator of transcription-3) is an oncogene that plays a critical role in regulating fundamental processes associated with malignant transformation and cell survival. It participates in oncogenesis through upregulation of genes encoding apoptosis inhibitors (Bcl-xL) and cell cycle regulators (cyclin D1). The expression of Stat3, Bcl-xL and cyclin D1 protein has not been investigated in extramammary Paget disease (EMPD). Objectives: To study the expression of phosphorylated Stat3 (p-Stat3), Bcl-xL and cyclin D1 protein in EMPD and to evaluate the relationships among them. Methods: Thirty-six tissue samples from 34 patients with primary EMPD were subjected to immunohistochemical staining for p-Stat3, cyclin D1 and Bcl-xL. Results: Thirty-five of 36 specimens were clearly positive for p-Stat3 in EMPD, while 30 of 36 and 32 of 36 were positive for cyclin D1 and Bcl-xL expression, respectively. In all of four invasive EMPD specimens, strong and frequent expression of these three molecules was evident; moreover, two invasive EMPD specimens with lymph nodal metastasis showed very strong nuclear and membranous p-Stat3 staining. Two metastatic lymph node specimens showed very strong nuclear and local membrane p-Stat3 staining. There were significant correlations between p-Stat3 and cyclin D1 expression and between p-Stat3 and Bcl-xL expression. Conclusions: Our study shows that the expression of p-Stat3, cyclin D 1 and Bcl-xL may play a pivotal role in the pathogenesis of EMPD.

AB - Background: Stat3 (Signal transducer and activator of transcription-3) is an oncogene that plays a critical role in regulating fundamental processes associated with malignant transformation and cell survival. It participates in oncogenesis through upregulation of genes encoding apoptosis inhibitors (Bcl-xL) and cell cycle regulators (cyclin D1). The expression of Stat3, Bcl-xL and cyclin D1 protein has not been investigated in extramammary Paget disease (EMPD). Objectives: To study the expression of phosphorylated Stat3 (p-Stat3), Bcl-xL and cyclin D1 protein in EMPD and to evaluate the relationships among them. Methods: Thirty-six tissue samples from 34 patients with primary EMPD were subjected to immunohistochemical staining for p-Stat3, cyclin D1 and Bcl-xL. Results: Thirty-five of 36 specimens were clearly positive for p-Stat3 in EMPD, while 30 of 36 and 32 of 36 were positive for cyclin D1 and Bcl-xL expression, respectively. In all of four invasive EMPD specimens, strong and frequent expression of these three molecules was evident; moreover, two invasive EMPD specimens with lymph nodal metastasis showed very strong nuclear and membranous p-Stat3 staining. Two metastatic lymph node specimens showed very strong nuclear and local membrane p-Stat3 staining. There were significant correlations between p-Stat3 and cyclin D1 expression and between p-Stat3 and Bcl-xL expression. Conclusions: Our study shows that the expression of p-Stat3, cyclin D 1 and Bcl-xL may play a pivotal role in the pathogenesis of EMPD.

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SP - 926

EP - 932

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 5

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