Expression of prophenoloxidase mRNA during silkworm hemocyte development

Kohji Yamamoto, Masatoshi Yakiyama, Hiroshi Fujii, Takahiro Kusakabe, Katsumi Koga, Yoichi Aso, Masatsune Ishiguro

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Two clones encoding different prophenoloxidase isoforms were amplified by polymerase chain reaction of RNA from the hemocytes of an experimental strain of Bombyx mori. The nucleotide sequences of the clones and the deduced amino acid sequences were confirmed to be nearly identical to those of the orthologous clones previously obtained from a commercial race of B. mori. Northern blot hybridization using these clones as probes demonstrated that the prophenoloxidase mRNA in the hemocytes is expressed in a stage-specific manner during the final larval instar and pupal stage, showing a peak one day before pupation in males and on the day of pupation in females. A sexual difference was also observed when the content of prophenoloxidase protein in the hemolymph (including hemocytes) was measured by an enzyme-linked immunosorbent assay.

Original languageEnglish
Pages (from-to)1197-1202
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume64
Issue number6
DOIs
Publication statusPublished - Jan 1 2000

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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