Expression of prothymosin-α and c-myc mRNA in human gastric cancer

Kenji Shibuta, Masaki Mori, Koshi Mimori, Hiroshi Inoue, Hideaki Nakashima, Kinya Baba, Masaru Haraguchi, Nobuya Karimine, Hiroaki Ueo, Tsuyoshi Akiyoshi

Research output: Contribution to journalArticle

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Abstract

Prothymosin-α (PT-α) is a nuclear protein involved in cell proliferation. c-myc is implicated in the carcinogenesis of many human cancers. PT-α gene transcription is reported to be regulated by the c-myc gene in vitro. However, little has been reported on the PT-α and c-myc mRNA expressions in gastric cancer. We semi-quantitatively determined the PT-α and c-myc mRNA expressions in 60 pairs of gastric cancer tissue (T) and corresponding normal tissue (N) using the reverse transcriptase-polymerase chain reaction method. The average of T/N ratio was 1.20 for PT-α and 1.30 for c-myc. Cases demonstrating a T/N ratio of more than 1.0 were seen in 33 (55%) and 30 (50%) cases for PT-α and c-myc, respectively. No significant correlation was observed between either of these two mRNA expressions and any of the examined clinicopathologic factors for gastric cancer. However, a significant correlation was seen between the expressions of both genes (p < 0.0001). The findings support the hypothesis that, regarding human gastric cancer, the transcription of PT-α is considered to be under the control of c-myc gene, however, the value of these gene expressions do not reflect biological behavior.

Original languageEnglish
Pages (from-to)247-251
Number of pages5
JournalInternational Journal of Oncology
Volume9
Issue number2
Publication statusPublished - Aug 1 1996

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Stomach Neoplasms
Messenger RNA
myc Genes
Gene Expression
Nuclear Proteins
Reverse Transcriptase Polymerase Chain Reaction
Carcinogenesis
Cell Proliferation
Genes
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Shibuta, K., Mori, M., Mimori, K., Inoue, H., Nakashima, H., Baba, K., ... Akiyoshi, T. (1996). Expression of prothymosin-α and c-myc mRNA in human gastric cancer. International Journal of Oncology, 9(2), 247-251.

Expression of prothymosin-α and c-myc mRNA in human gastric cancer. / Shibuta, Kenji; Mori, Masaki; Mimori, Koshi; Inoue, Hiroshi; Nakashima, Hideaki; Baba, Kinya; Haraguchi, Masaru; Karimine, Nobuya; Ueo, Hiroaki; Akiyoshi, Tsuyoshi.

In: International Journal of Oncology, Vol. 9, No. 2, 01.08.1996, p. 247-251.

Research output: Contribution to journalArticle

Shibuta, K, Mori, M, Mimori, K, Inoue, H, Nakashima, H, Baba, K, Haraguchi, M, Karimine, N, Ueo, H & Akiyoshi, T 1996, 'Expression of prothymosin-α and c-myc mRNA in human gastric cancer', International Journal of Oncology, vol. 9, no. 2, pp. 247-251.
Shibuta, Kenji ; Mori, Masaki ; Mimori, Koshi ; Inoue, Hiroshi ; Nakashima, Hideaki ; Baba, Kinya ; Haraguchi, Masaru ; Karimine, Nobuya ; Ueo, Hiroaki ; Akiyoshi, Tsuyoshi. / Expression of prothymosin-α and c-myc mRNA in human gastric cancer. In: International Journal of Oncology. 1996 ; Vol. 9, No. 2. pp. 247-251.
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