Expression of recombinant viscum album coloratum lectin B-chain in the silkworm expression system and evaluation of antioxidant activity

Sun Mee Hong, Ji Hyun Choi, Sun Jung Jo, Seong Kyu Song, Man Lee, Takahiro Kusakabe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Korean mistletoe lectins (KMLs) possess many biological activities, including anti-tumor, apoptosis-inducing, anti-metastatic, and anti-angiogenic activities. Recombinant KML-1A (rKML-1A) has been previously expressed in soluble form in Escherichia coli cells. However, the expression of rKML-1B in soluble form remains to be accomplished. In this study, we describe the production, purification, and characterization of recombinant KML-1B by using a baculovirus expression system, which employs silkworm larvae and pupae as hosts. Approximately 495 and 702 µg/mL of a 36-kDa rKML-1B protein were produced without a signal peptide by silkworm larvae and pupae, respectively. Treatment of the recombinant protein with N- or O-deglycosylase led to a decrease in its molecular mass, indicating the N-and/or O-glycosylation of rKML-1B. Purified rKML-1B displayed radical scavenging activities toward 1,1-diphenyl-2-picrylhydrazyl and 2,2′-amino-di-[3-ethylbenzthiazoline sulfonate], with a half maximal inhibitory concentration (IC50) of approximately 95 µg/mL. This activity was discovered to be stable at 65°C. To our knowledge, this is the first report detailing the biological activity of plant-derived, pure rKML-1B.

Original languageEnglish
Pages (from-to)515-522
Number of pages8
JournalBiotechnology and Bioprocess Engineering
Volume20
Issue number3
DOIs
Publication statusPublished - Jun 25 2015

Fingerprint

Viscum album
Mistletoe
Bombyx
Antioxidants
Lectins
Pupa
Bioactivity
Larva
Glycosylation
Recombinant proteins
Baculoviridae
Scavenging
Molecular mass
Cell death
Protein Sorting Signals
Recombinant Proteins
Escherichia coli
Inhibitory Concentration 50
Purification
Tumors

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

Cite this

Expression of recombinant viscum album coloratum lectin B-chain in the silkworm expression system and evaluation of antioxidant activity. / Hong, Sun Mee; Choi, Ji Hyun; Jo, Sun Jung; Song, Seong Kyu; Lee, Man; Kusakabe, Takahiro.

In: Biotechnology and Bioprocess Engineering, Vol. 20, No. 3, 25.06.2015, p. 515-522.

Research output: Contribution to journalArticle

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