Expression of recombination-activating gene in mature peripheral T cells in Peyer's patch

Eisuke Kondo, Hiroshi Wakao, Haruhiko Koseki, Toshitada Takemori, Satoshi Kojo, Michishige Harada, Minako Takahashi, Sakura Sakata, Chiori Shimizu, Toshihiro Ito, Toshinori Nakayama, Masaru Taniguchi

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Abstract

Recombination-activating gene (RAG) 1 and 2 are essential for the gene rearrangement of antigen receptors of both T and B cells. To investigate RAG gene expression in peripheral lymphoid organs other than the thymus and bone marrow, we established mice in which a green fluorescent protein (GFP) gene is knocked-in the RAG2 gene locus (RAG2-GFP mice). In the thymus and bone marrow of heterozygous RAG2-GFP mice, as expected, GFP expression was detected in the appropriate stages of developing T and B cells. Interestingly, only a fraction of Thy-1.2+ cells in the Peyer's patch were found to be GFP+ amongst the peripheral lymphoid organs. The GFP+ cells expressed high levels of surface TCRβ and CD3, suggesting mature T cells with rearranged TCRαβ. However, they showed activated/memory phenotypes, i.e. CD45RBlow, CD69high, CD44high and CD62Llow, and belonged to a CD4+CD8+ population expressing c-kit, IL-7R and pTα characteristic of immature developing lymphocytes. Moreover, RAG+ Peyer's patch T cells seem to be of thymic origin as judged by their expression of CD8αβ. These results show that there exists a fraction of mature T cells expressing RAG genes in the Peyer's patch, implying a potential for a secondary rearrangement of TCR in extrathymic tissues.

Original languageEnglish
Pages (from-to)393-402
Number of pages10
JournalInternational immunology
Volume15
Issue number3
DOIs
Publication statusPublished - Mar 1 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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    Kondo, E., Wakao, H., Koseki, H., Takemori, T., Kojo, S., Harada, M., Takahashi, M., Sakata, S., Shimizu, C., Ito, T., Nakayama, T., & Taniguchi, M. (2003). Expression of recombination-activating gene in mature peripheral T cells in Peyer's patch. International immunology, 15(3), 393-402. https://doi.org/10.1093/intimm/dxg040