Expression of ribonuclease T1 in escherichia coli and rapid purification of the enzyme

Rainer Quaas; Hans Peter Grunert; Makoto Kimura; Ulrich Hahn

doi:10.1080/07328318808056297

Expression of ribonuclease T1 in escherichia coli and rapid purification of the enzyme

Rainer Quaas, Hans Peter Grunert, Makoto Kimura, Ulrich Hahn

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

An Escherichia coli clone secreting native ribonuclease T1 into the periplasm has been constructed. In a two step purification procedure 20 mg of pure enzyme can be isolated starting from 1 litre of liquid culture.

Original language	English
Pages (from-to)	619-623
Number of pages	5
Journal	Nucleosides and Nucleotides
Volume	7
Issue number	5-6
DOIs	https://doi.org/10.1080/07328318808056297
Publication status	Published - Oct 1 1988

All Science Journal Classification (ASJC) codes

Biochemistry
Genetics

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title = "Expression of ribonuclease T1 in escherichia coli and rapid purification of the enzyme",

abstract = "An Escherichia coli clone secreting native ribonuclease T1 into the periplasm has been constructed. In a two step purification procedure 20 mg of pure enzyme can be isolated starting from 1 litre of liquid culture.",

author = "Rainer Quaas and Grunert, {Hans Peter} and Makoto Kimura and Ulrich Hahn",

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AU - Kimura, Makoto

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