Expression of ribonuclease T1 in escherichia coli and rapid purification of the enzyme

Rainer Quaas, Hans Peter Grunert, Makoto Kimura, Ulrich Hahn

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

An Escherichia coli clone secreting native ribonuclease T1 into the periplasm has been constructed. In a two step purification procedure 20 mg of pure enzyme can be isolated starting from 1 litre of liquid culture.

Original languageEnglish
Pages (from-to)619-623
Number of pages5
JournalNucleosides and Nucleotides
Volume7
Issue number5-6
DOIs
Publication statusPublished - Oct 1 1988

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics

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