Expression of ribonuclease T1 in escherichia coli and rapid purification of the enzyme

Rainer Quaas; Hans Peter Grunert; Makoto Kimura; Ulrich Hahn

doi:10.1080/07328318808056297

Expression of ribonuclease T1 in escherichia coli and rapid purification of the enzyme

Rainer Quaas, Hans Peter Grunert, Makoto Kimura, Ulrich Hahn

Molecular Biosciences

Research output: Contribution to journal › Article

31 Citations (Scopus)

Abstract

An Escherichia coli clone secreting native ribonuclease T1 into the periplasm has been constructed. In a two step purification procedure 20 mg of pure enzyme can be isolated starting from 1 litre of liquid culture.

Original language	English
Pages (from-to)	619-623
Number of pages	5
Journal	Nucleosides and Nucleotides
Volume	7
Issue number	5-6
DOIs	https://doi.org/10.1080/07328318808056297
Publication status	Published - Oct 1 1988

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All Science Journal Classification (ASJC) codes

Biochemistry
Genetics

Cite this

Quaas, R., Grunert, H. P., Kimura, M., & Hahn, U. (1988). Expression of ribonuclease T1 in escherichia coli and rapid purification of the enzyme. Nucleosides and Nucleotides, 7(5-6), 619-623. https://doi.org/10.1080/07328318808056297

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Access to Document

10.1080/07328318808056297