@article{12968e54816d4c9fae169ab999ac9072,
title = "Expression of ribonuclease T1 in escherichia coli and rapid purification of the enzyme",
abstract = "An Escherichia coli clone secreting native ribonuclease T1 into the periplasm has been constructed. In a two step purification procedure 20 mg of pure enzyme can be isolated starting from 1 litre of liquid culture.",
author = "Rainer Quaas and Grunert, {Hans Peter} and Makoto Kimura and Ulrich Hahn",
note = "Funding Information: Acknowledgements: We thank Wolfram Saenger and Udo Heinemann for reading the manuscript and Sabine Schultze for synthesizing the mutagenesis primer. This work was supported by the Deutsche Forschungsgemeinschaft (grant Ha 1366/1-2).",
year = "1988",
month = oct,
day = "1",
doi = "10.1080/07328318808056297",
language = "English",
volume = "7",
pages = "619--623",
journal = "Nucleosides and Nucleotides",
issn = "0732-8311",
publisher = "Taylor and Francis Ltd.",
number = "5-6",
}