Abstract
Since the substantia nigra receives abundant substance P innervations but lacks clear evidences about a presence of substance P receptors, expressions for mRNA and protein of substance P receptors were investigated in the rat to resolve this mismatch. Expression levels of substance P receptors mRNA in the substantia nigra pars compacta and reticulata were 37.7 and 24.1% of those in the striatum, respectively, by reverse transcription-polymerase chain reaction (RT-PCR). Substance P receptors mRNA was found in dopamine neurons of the substantia nigra pars compacta by single cell RT-PCR. Ca. 90% of dopamine neurons in the substantia nigra pars compacta were immunoreactive to anti-substance P receptor antibody in the colchicine treated rats. These are the first direct evidence for the existence of substance P receptors in dopamine neurons of the substantia nigra pars compacta.
Original language | English |
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Pages (from-to) | 183-198 |
Number of pages | 16 |
Journal | Molecular Brain Research |
Volume | 54 |
Issue number | 2 |
DOIs | |
Publication status | Published - Mar 1 1998 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cellular and Molecular Neuroscience