Abstract
Since the substantia nigra receives abundant substance P innervations but lacks clear evidences about a presence of substance P receptors, expressions for mRNA and protein of substance P receptors were investigated in the rat to resolve this mismatch. Expression levels of substance P receptors mRNA in the substantia nigra pars compacta and reticulata were 37.7 and 24.1% of those in the striatum, respectively, by reverse transcription-polymerase chain reaction (RT-PCR). Substance P receptors mRNA was found in dopamine neurons of the substantia nigra pars compacta by single cell RT-PCR. Ca. 90% of dopamine neurons in the substantia nigra pars compacta were immunoreactive to anti-substance P receptor antibody in the colchicine treated rats. These are the first direct evidence for the existence of substance P receptors in dopamine neurons of the substantia nigra pars compacta.
| Original language | English |
|---|---|
| Pages (from-to) | 183-198 |
| Number of pages | 16 |
| Journal | Molecular Brain Research |
| Volume | 54 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 1 1998 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cellular and Molecular Neuroscience
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Expression of substance P receptor in the substantia nigra. / Futami, T.; Hatanaka, Y.; Matsushita, K.; Furuya, Shigeki.
In: Molecular Brain Research, Vol. 54, No. 2, 01.03.1998, p. 183-198.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of substance P receptor in the substantia nigra
AU - Futami, T.
AU - Hatanaka, Y.
AU - Matsushita, K.
AU - Furuya, Shigeki
PY - 1998/3/1
Y1 - 1998/3/1
N2 - Since the substantia nigra receives abundant substance P innervations but lacks clear evidences about a presence of substance P receptors, expressions for mRNA and protein of substance P receptors were investigated in the rat to resolve this mismatch. Expression levels of substance P receptors mRNA in the substantia nigra pars compacta and reticulata were 37.7 and 24.1% of those in the striatum, respectively, by reverse transcription-polymerase chain reaction (RT-PCR). Substance P receptors mRNA was found in dopamine neurons of the substantia nigra pars compacta by single cell RT-PCR. Ca. 90% of dopamine neurons in the substantia nigra pars compacta were immunoreactive to anti-substance P receptor antibody in the colchicine treated rats. These are the first direct evidence for the existence of substance P receptors in dopamine neurons of the substantia nigra pars compacta.
AB - Since the substantia nigra receives abundant substance P innervations but lacks clear evidences about a presence of substance P receptors, expressions for mRNA and protein of substance P receptors were investigated in the rat to resolve this mismatch. Expression levels of substance P receptors mRNA in the substantia nigra pars compacta and reticulata were 37.7 and 24.1% of those in the striatum, respectively, by reverse transcription-polymerase chain reaction (RT-PCR). Substance P receptors mRNA was found in dopamine neurons of the substantia nigra pars compacta by single cell RT-PCR. Ca. 90% of dopamine neurons in the substantia nigra pars compacta were immunoreactive to anti-substance P receptor antibody in the colchicine treated rats. These are the first direct evidence for the existence of substance P receptors in dopamine neurons of the substantia nigra pars compacta.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032031799&partnerID=8YFLogxK
U2 - 10.1016/S0169-328X(97)00307-0
DO - 10.1016/S0169-328X(97)00307-0
M3 - Article
C2 - 9555006
AN - SCOPUS:0032031799
VL - 54
SP - 183
EP - 198
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -