Expression of substance P receptor in the substantia nigra

T. Futami, Y. Hatanaka, K. Matsushita, Shigeki Furuya

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Since the substantia nigra receives abundant substance P innervations but lacks clear evidences about a presence of substance P receptors, expressions for mRNA and protein of substance P receptors were investigated in the rat to resolve this mismatch. Expression levels of substance P receptors mRNA in the substantia nigra pars compacta and reticulata were 37.7 and 24.1% of those in the striatum, respectively, by reverse transcription-polymerase chain reaction (RT-PCR). Substance P receptors mRNA was found in dopamine neurons of the substantia nigra pars compacta by single cell RT-PCR. Ca. 90% of dopamine neurons in the substantia nigra pars compacta were immunoreactive to anti-substance P receptor antibody in the colchicine treated rats. These are the first direct evidence for the existence of substance P receptors in dopamine neurons of the substantia nigra pars compacta.

Original languageEnglish
Pages (from-to)183-198
Number of pages16
JournalMolecular Brain Research
Volume54
Issue number2
DOIs
Publication statusPublished - Mar 1 1998
Externally publishedYes

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Neurokinin-1 Receptors
Substantia Nigra
Dopaminergic Neurons
Messenger RNA
Reverse Transcription
Polymerase Chain Reaction
Colchicine
Substance P
Pars Compacta
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Expression of substance P receptor in the substantia nigra. / Futami, T.; Hatanaka, Y.; Matsushita, K.; Furuya, Shigeki.

In: Molecular Brain Research, Vol. 54, No. 2, 01.03.1998, p. 183-198.

Research output: Contribution to journalArticle

Futami, T. ; Hatanaka, Y. ; Matsushita, K. ; Furuya, Shigeki. / Expression of substance P receptor in the substantia nigra. In: Molecular Brain Research. 1998 ; Vol. 54, No. 2. pp. 183-198.
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