Expression of sucrose synthase in sweet potato

Kazuyuki Saitou, Takuya Araki, Waichi Agata, Fumitake Kubota, Kaoru Nakayama

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

PCR amplification of cDNA prepared from the poly(A)+ RNA of tuberous roots of sweet potato, using degenerate oligonucleotide primers based on highly conserved regions among sucrose synthase (EG 2. 4. 1. 13) reported previously, yielded a cDNA of 1,191 bp (IBSUS). The nucleotide sequence of IBSUS exhibited a high degree of homology with the corresponding regions of the potato sucrose synthase cDNA sequences (82 and 86% of identical nucleotides). Less homology (76~77%) was found in the monocotyledonous sequences (maize, rice and barley). The activity of sucrose synthase in the tuberous roots of sweet potato was higher than that in other parts, namely, leaf blades, petioles, stolons and fibrous roots. The activity of sucrose synthase in roots increased markedly following an increase in sucrose during development of the tuberous roots. Northern blot analysis using IBSUS as a probe revealed that a signal of sucrose synthase mRNA with a size of approximately 2.4 kb was present in petioles, stolons, fibrous roots and tuberous roots, and the levels of sucrose synthase mRNA in different parts and in the roots during development of tuberous roots were highly correlated with enzymatic activities. In petioles, an increase in sucrose concentration led to an increase in the activity of sucrose synthase.

Original languageEnglish
Pages (from-to)624-631
Number of pages8
JournalJapanese Journal of Crop Science
Volume66
Issue number4
DOIs
Publication statusPublished - Jan 1 1997

Fingerprint

Ipomoea batatas
sucrose synthase
sweet potatoes
petioles
fibrous roots
stolons
Complementary DNA
Messenger RNA
Sucrose
sucrose
DNA Primers
DNA primers
Hordeum
messenger RNA
Solanum tuberosum
leaf blade
Northern blotting
Northern Blotting
Zea mays
Nucleotides

All Science Journal Classification (ASJC) codes

  • Food Science
  • Agronomy and Crop Science
  • Genetics

Cite this

Expression of sucrose synthase in sweet potato. / Saitou, Kazuyuki; Araki, Takuya; Agata, Waichi; Kubota, Fumitake; Nakayama, Kaoru.

In: Japanese Journal of Crop Science, Vol. 66, No. 4, 01.01.1997, p. 624-631.

Research output: Contribution to journalArticle

Saitou, K, Araki, T, Agata, W, Kubota, F & Nakayama, K 1997, 'Expression of sucrose synthase in sweet potato', Japanese Journal of Crop Science, vol. 66, no. 4, pp. 624-631. https://doi.org/10.1626/jcs.66.624
Saitou, Kazuyuki ; Araki, Takuya ; Agata, Waichi ; Kubota, Fumitake ; Nakayama, Kaoru. / Expression of sucrose synthase in sweet potato. In: Japanese Journal of Crop Science. 1997 ; Vol. 66, No. 4. pp. 624-631.
@article{afa110cc85f747fab9f512a9fe61cc82,
title = "Expression of sucrose synthase in sweet potato",
abstract = "PCR amplification of cDNA prepared from the poly(A)+ RNA of tuberous roots of sweet potato, using degenerate oligonucleotide primers based on highly conserved regions among sucrose synthase (EG 2. 4. 1. 13) reported previously, yielded a cDNA of 1,191 bp (IBSUS). The nucleotide sequence of IBSUS exhibited a high degree of homology with the corresponding regions of the potato sucrose synthase cDNA sequences (82 and 86{\%} of identical nucleotides). Less homology (76~77{\%}) was found in the monocotyledonous sequences (maize, rice and barley). The activity of sucrose synthase in the tuberous roots of sweet potato was higher than that in other parts, namely, leaf blades, petioles, stolons and fibrous roots. The activity of sucrose synthase in roots increased markedly following an increase in sucrose during development of the tuberous roots. Northern blot analysis using IBSUS as a probe revealed that a signal of sucrose synthase mRNA with a size of approximately 2.4 kb was present in petioles, stolons, fibrous roots and tuberous roots, and the levels of sucrose synthase mRNA in different parts and in the roots during development of tuberous roots were highly correlated with enzymatic activities. In petioles, an increase in sucrose concentration led to an increase in the activity of sucrose synthase.",
author = "Kazuyuki Saitou and Takuya Araki and Waichi Agata and Fumitake Kubota and Kaoru Nakayama",
year = "1997",
month = "1",
day = "1",
doi = "10.1626/jcs.66.624",
language = "English",
volume = "66",
pages = "624--631",
journal = "Japanese Journal of Crop Science",
issn = "1349-0990",
publisher = "日本作物学会",
number = "4",

}

TY - JOUR

T1 - Expression of sucrose synthase in sweet potato

AU - Saitou, Kazuyuki

AU - Araki, Takuya

AU - Agata, Waichi

AU - Kubota, Fumitake

AU - Nakayama, Kaoru

PY - 1997/1/1

Y1 - 1997/1/1

N2 - PCR amplification of cDNA prepared from the poly(A)+ RNA of tuberous roots of sweet potato, using degenerate oligonucleotide primers based on highly conserved regions among sucrose synthase (EG 2. 4. 1. 13) reported previously, yielded a cDNA of 1,191 bp (IBSUS). The nucleotide sequence of IBSUS exhibited a high degree of homology with the corresponding regions of the potato sucrose synthase cDNA sequences (82 and 86% of identical nucleotides). Less homology (76~77%) was found in the monocotyledonous sequences (maize, rice and barley). The activity of sucrose synthase in the tuberous roots of sweet potato was higher than that in other parts, namely, leaf blades, petioles, stolons and fibrous roots. The activity of sucrose synthase in roots increased markedly following an increase in sucrose during development of the tuberous roots. Northern blot analysis using IBSUS as a probe revealed that a signal of sucrose synthase mRNA with a size of approximately 2.4 kb was present in petioles, stolons, fibrous roots and tuberous roots, and the levels of sucrose synthase mRNA in different parts and in the roots during development of tuberous roots were highly correlated with enzymatic activities. In petioles, an increase in sucrose concentration led to an increase in the activity of sucrose synthase.

AB - PCR amplification of cDNA prepared from the poly(A)+ RNA of tuberous roots of sweet potato, using degenerate oligonucleotide primers based on highly conserved regions among sucrose synthase (EG 2. 4. 1. 13) reported previously, yielded a cDNA of 1,191 bp (IBSUS). The nucleotide sequence of IBSUS exhibited a high degree of homology with the corresponding regions of the potato sucrose synthase cDNA sequences (82 and 86% of identical nucleotides). Less homology (76~77%) was found in the monocotyledonous sequences (maize, rice and barley). The activity of sucrose synthase in the tuberous roots of sweet potato was higher than that in other parts, namely, leaf blades, petioles, stolons and fibrous roots. The activity of sucrose synthase in roots increased markedly following an increase in sucrose during development of the tuberous roots. Northern blot analysis using IBSUS as a probe revealed that a signal of sucrose synthase mRNA with a size of approximately 2.4 kb was present in petioles, stolons, fibrous roots and tuberous roots, and the levels of sucrose synthase mRNA in different parts and in the roots during development of tuberous roots were highly correlated with enzymatic activities. In petioles, an increase in sucrose concentration led to an increase in the activity of sucrose synthase.

UR - http://www.scopus.com/inward/record.url?scp=0031468739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031468739&partnerID=8YFLogxK

U2 - 10.1626/jcs.66.624

DO - 10.1626/jcs.66.624

M3 - Article

AN - SCOPUS:0031468739

VL - 66

SP - 624

EP - 631

JO - Japanese Journal of Crop Science

JF - Japanese Journal of Crop Science

SN - 1349-0990

IS - 4

ER -