Expression of T cell antigen receptor genes in the thymus of irradiated mice after bone marrow transplantation

G. Matsuzaki, Y. Yoshikai, K. Kishihara, K. Nomoto

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Sequential appearance of the expression of T cell antigen receptor genes was investigated in the thymus of irradiated mice at the early stage after transplantation of Thy-1 congeneic H-2 compatible allogeneic bone marrow cells. The first cells to repopulate the thymus on day 7 after bone marrow transplantation were intrathymic radioresistant T cell precursors, which expanded mainly to CD4+CD8+ host-type thymocytes by day 14. A high level of γ gene expression but a much reduced level of α and β gene expression were detected in the host-type thymocytes on day 7. During regeneration of these cells, γ-chain messages fell to low level and α and β mRNA levels increased. The thymus of the recipients began to be repopulated by donor-derived T cells about 2 wk after bone marrow transplantation and was almost completely replaced by the third week. An ordered expression of γ then β and α-chain gene transcript was also observed in the donor-type thymocytes at the early stage after bone marrow transplantation. The use of thymocytes at an early stage in whole-body irradiated bone marrow chimera provides a pertinent source for investigating the molecular mechanism of T cell differentiation in adult thymus.

    Original languageEnglish
    Pages (from-to)385-387
    Number of pages3
    JournalJournal of Immunology
    Volume140
    Issue number2
    Publication statusPublished - Jan 1 1988

    Fingerprint

    T-Cell Receptor Genes
    Thymocytes
    T-Cell Antigen Receptor
    Bone Marrow Transplantation
    Thymus Gland
    T-Lymphoid Precursor Cells
    T-Lymphocytes
    Gene Expression
    Bone Marrow Cells
    Regeneration
    Cell Differentiation
    Transplantation
    Bone Marrow
    Messenger RNA
    Genes

    All Science Journal Classification (ASJC) codes

    • Immunology

    Cite this

    Expression of T cell antigen receptor genes in the thymus of irradiated mice after bone marrow transplantation. / Matsuzaki, G.; Yoshikai, Y.; Kishihara, K.; Nomoto, K.

    In: Journal of Immunology, Vol. 140, No. 2, 01.01.1988, p. 385-387.

    Research output: Contribution to journalArticle

    Matsuzaki, G, Yoshikai, Y, Kishihara, K & Nomoto, K 1988, 'Expression of T cell antigen receptor genes in the thymus of irradiated mice after bone marrow transplantation', Journal of Immunology, vol. 140, no. 2, pp. 385-387.
    Matsuzaki, G. ; Yoshikai, Y. ; Kishihara, K. ; Nomoto, K. / Expression of T cell antigen receptor genes in the thymus of irradiated mice after bone marrow transplantation. In: Journal of Immunology. 1988 ; Vol. 140, No. 2. pp. 385-387.
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