Expression of tetra-spans transmembrane family (CD9, CD37, CD53, CD63, CD81 and CD82) in normal and neoplastic human keratinocytes: An association of CD9 with α3β1 integrin

H. Okochi, M. Kato, K. Nashiro, O. Yoshie, K. Miyazono, Masutaka Furue

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Tetra-spans transmembrane family (TSTF) members (CD9, CD37, CD53, CD63, CD81 and CD82) have potent effects on cell growth, motility and adhesion in various cells. However, little is known about their expression in human skin. Using immunohistological techniques, we have studied the localization of all six members of TSTF in normal and carcinomatous human keratinocytes. CD9, CD81 and CD82 were expressed in the entire living layers of the epidermis. Their staining pattern was quite similar, and was mainly intercellular with occasional intracellular immunoreactivity. CD53 expression was confined to the intercellular spaces of the upper spinous or granular layer in the normal epidermis. No clear-cut expression of CD63 could be detected in the epidermis. CD 37 was not detected at all. Cultured human keratinocytes also expressed CD9, CD81 and CD82 at the surface membrane of cell-cell boundaries. Expression of CD37 and CD53 was negative in cultured keratinocytes, while CD63 was clearly localized in the cytoplasmic lysosomes. An immunoprecipitation assay revealed that α3β1 integrin is molecularly associated with CD9. The expression of CD9, CD81 and CD82 was markedly down- regulated in basal cell carcinoma but not in Bowen's disease. The abundant and differential expression of TSTF molecules and the selective association of CD9 with α3β1 integrin suggest that the TSTF molecules may be involved in the regulation of epidermal differentiation and integrity in vivo.

Original languageEnglish
Pages (from-to)856-863
Number of pages8
JournalBritish Journal of Dermatology
Volume137
Issue number6
DOIs
Publication statusPublished - Jan 1 1997
Externally publishedYes

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Keratinocytes
Integrins
Epidermis
Bowen's Disease
Basal Cell Carcinoma
Extracellular Space
Lysosomes
Immunoprecipitation
Cell Adhesion
Cell Movement
Cell Membrane
Staining and Labeling
Skin
Growth
Neoplasms

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Expression of tetra-spans transmembrane family (CD9, CD37, CD53, CD63, CD81 and CD82) in normal and neoplastic human keratinocytes : An association of CD9 with α3β1 integrin. / Okochi, H.; Kato, M.; Nashiro, K.; Yoshie, O.; Miyazono, K.; Furue, Masutaka.

In: British Journal of Dermatology, Vol. 137, No. 6, 01.01.1997, p. 856-863.

Research output: Contribution to journalArticle

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