Expression of the α 2 -macroglobulin-encoding gene in rat brain and cultured astrocytes

Masakazu Higuchi, Takashi Ito, Yoshinori Imai, Toru Iwaki, Masahira Hattori, Shinichi Kohsaka, Yoshiyuki Niho, Yoshiyuki Sakaki

Research output: Contribution to journalArticle

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Abstract

The α 2 -macroglobulin (α 2 M), a protease inhibitor, is a major acute-phase protein in rats, and is produced in the liver during acute inflammation. Recently, it has been demonstrated that α 2 M is also produced by cultured astrocytes from newborn rat brain and has neurite-promoting activity. Here, we found that the expression of the α 2 M gene was significantly enhanced in the brain following intraperitoneal injection of the neurotoxicant, kainic acid (KA), suggesting that α 2 M acts as an acute-phase protein in the brain, as in the case of the liver, and may be involved in neural repair processes. Expression of α 2 M in cultured astrocytes was shown to be stimulated by interleukin-6 (IL-6) and/or leukemia inhibitory factor (LIF) in the presence of glucocorticoid. The amount of mRNAs for IL-6 and LIF increased in the brain of KA-injected rats prior to α 2 M induction. These results strongly suggested that IL-6 and LIF are involved in α 2 M induction in the brain, as in the case of the liver. Analysis of the cis-acting element(s) and the trans-acting factor(s) suggested that the regulatory mechanism for α 2 M expression in astrocytes was similar to that in inflamed liver.

Original languageEnglish
Pages (from-to)155-162
Number of pages8
JournalGene
Volume141
Issue number2
DOIs
Publication statusPublished - Apr 20 1994

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Macroglobulins
Astrocytes
Leukemia Inhibitory Factor
Brain
Interleukin-6
Acute-Phase Proteins
Genes
Kainic Acid
Liver
Trans-Activators
Neurites
Protease Inhibitors
Intraperitoneal Injections
Glucocorticoids
Inflammation
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Genetics

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Expression of the α 2 -macroglobulin-encoding gene in rat brain and cultured astrocytes . / Higuchi, Masakazu; Ito, Takashi; Imai, Yoshinori; Iwaki, Toru; Hattori, Masahira; Kohsaka, Shinichi; Niho, Yoshiyuki; Sakaki, Yoshiyuki.

In: Gene, Vol. 141, No. 2, 20.04.1994, p. 155-162.

Research output: Contribution to journalArticle

Higuchi, M, Ito, T, Imai, Y, Iwaki, T, Hattori, M, Kohsaka, S, Niho, Y & Sakaki, Y 1994, ' Expression of the α 2 -macroglobulin-encoding gene in rat brain and cultured astrocytes ', Gene, vol. 141, no. 2, pp. 155-162. https://doi.org/10.1016/0378-1119(94)90565-7
Higuchi, Masakazu ; Ito, Takashi ; Imai, Yoshinori ; Iwaki, Toru ; Hattori, Masahira ; Kohsaka, Shinichi ; Niho, Yoshiyuki ; Sakaki, Yoshiyuki. / Expression of the α 2 -macroglobulin-encoding gene in rat brain and cultured astrocytes In: Gene. 1994 ; Vol. 141, No. 2. pp. 155-162.
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