Expression of the c-myc and c-fos genes as a potential indicator of late radiation damage to the kidney

Makoto Otsuka, Masamitsu Hatakenaka, Kosei Ishigami, Kouji Masuda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose: To evaluate proliferative response according to c-myc and c-fos gene expression as an early indicator of late radiation damage to the kidney. Methods and Materials: c-myc and c-fos gene expression was measured in the irradiated kidney of C3H/HeSlc mice using reverse transcriptase-polymerase chain reaction (RT-PCR) 24 h after 9, 12, and 15 Gy as well as 2 and 7 days after 15 Gy. In a second experiment, 12 Gy was administered to the right kidney plus the lower half of left kidney. The mice underwent left nephrectomy 24 h after, and the correlation between increased gene expression and elevation of the blood urea nitrogen (BUN) level representing late functional damage was investigated. Results: c-myc and c-fos expression increased in 1 of 10 controls and in 8 of 10 and 7 of 10 mice that received 15 Gy, respectively. Expression increased dose-dependently from 9 to 15 Gy. In mice having an increased BUN level, 7 of 10 and 8 of 10 were positive for c-myc and c-fos, respectively. All 6 mice having an unchanged BUN level were negative for c-fos, while 5 were negative for c-myc. Conclusion: Measurement of c-myc and c-fos gene expression is potentially an early indicator of late radiation damage to the kidney.

Original languageEnglish
Pages (from-to)169-173
Number of pages5
JournalInternational Journal of Radiation Oncology Biology Physics
Volume49
Issue number1
DOIs
Publication statusPublished - Jan 1 2001

Fingerprint

fos Genes
kidneys
radiation damage
genes
gene expression
mice
Radiation
Blood Urea Nitrogen
Kidney
ureas
blood
Gene Expression
nitrogen
polymerase chain reaction
Inbred C3H Mouse
Reverse Transcriptase Polymerase Chain Reaction
Nephrectomy
damage
dosage

All Science Journal Classification (ASJC) codes

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Expression of the c-myc and c-fos genes as a potential indicator of late radiation damage to the kidney. / Otsuka, Makoto; Hatakenaka, Masamitsu; Ishigami, Kosei; Masuda, Kouji.

In: International Journal of Radiation Oncology Biology Physics, Vol. 49, No. 1, 01.01.2001, p. 169-173.

Research output: Contribution to journalArticle

Otsuka, Makoto ; Hatakenaka, Masamitsu ; Ishigami, Kosei ; Masuda, Kouji. / Expression of the c-myc and c-fos genes as a potential indicator of late radiation damage to the kidney. In: International Journal of Radiation Oncology Biology Physics. 2001 ; Vol. 49, No. 1. pp. 169-173.
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