Expression of the human MHC, HLA-DQw6 genes alters the immune response in C57BL/6 mice

Y. Nishimura, T. Iwanaga, T. Inamitsu, Y. Yanagawa, M. Yasunami, A. Kimura, K. Hirokawa, Takehiko Sasazuki

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In an attempt to obtain direct evidence for the critical role of HLA class II molecules in regulating the immune response, genomic genes for α- and β-chains of HLA-DQw6 from HLA-Dw12 haplotype were introduced into the C57BL/6 (B6) strain of mouse and a line of HLA-DQw6 transgenic mouse was obtained. Tissue specificity of the expression of the transgenes was much the same as that of murine L-Ab genes. DQw6 molecules were expressed on B cells and macrophages in spleen cells and about 30 to 40% of the I-Ab+ spleen cells were positive for DQw6. The HLA-DQw6 transgenic B6 mouse became tolerant to the DQw6 molecules, as evidenced by the MLR and antibody production specific to the DQw6 molecules. The HLA-DQw6 transgenic B6 mouse showed a strong immune response to streptococcal cell wall antigen (SCW), whereas the B6 mouse was a low responder to SCW. The SCW-specific T cell line was established from the transgenic mouse and this T cell line recognized SCW in the context of HLA-DQw6 molecules expressed on the mouse L cell transfectant or on human monocytes. The proliferative response to SCW of primed lymph node T cells and the SCW-specific T cell line derived from the transgenic mice was inhibited by anti-HLA-DQ mAb. Thus, it is clear that the HLA-DQw6 genes acted as major histocompatibility genes in these transgenic mice.

Original languageEnglish
Pages (from-to)353-360
Number of pages8
JournalJournal of Immunology
Volume145
Issue number1
Publication statusPublished - 1990

All Science Journal Classification (ASJC) codes

  • Immunology

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