Expression of the mRNA for types I and II interleukin-1 receptors in dental tissues of mice during tooth development

L. X. Xu, Toshio Kukita, H. Yu, Y. Nakano, T. Koga

Research output: Contribution to journalArticle

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Abstract

Interleukin-1 (IL-1) can exert its pleiotropic effects on nearly every tissue by binding to its cognate receptor. Two types of IL-1 receptors have been identified. A large number of cell types have been shown to possess IL- 1 receptors in vitro and in vivo, but few studies have addressed the question of expression in dental tissues in vivo. Using in situ hybridization in normal newborn, young and adult mice, we have examined the cellular distribution of both types of IL-1 receptors in dental tissues. In the ameloblast layer of incisors and molars, the mRNA for the type I IL-1 receptor (IL-1RI) and the type II IL-1 receptor (IL-1RII) was detected at the presecretory stage. The expression level markedly increased and remained during amelogenesis at the secretory stage. At the maturation stage, however, the transcripts for both IL-1RI and -II mRNA disappeared. Expression of IL- 1RI and -II mRNA was also observed in odontoblasts after crown morphogenesis had been completed, and continued in these cells during dentinogenesis. No transcripts were detected in stratum intermedium cells and other cells in dental follicle, stellate reticulum, dental papilla, or pulp. Additionally, both types of IL-1R mRNA were also detected in osteoclasts on surfaces of alveolar bone. These results demonstrated for the first time that enamel- secreting ameloblasts and dentine-secreting odontoblasts express IL-1RI and - II mRNA, suggesting that IL-1 plays a regulatory role in the function of ameloblasts and odontoblasts during tooth development of mice.

Original languageEnglish
Pages (from-to)351-356
Number of pages6
JournalCalcified Tissue International
Volume63
Issue number4
DOIs
Publication statusPublished - Oct 1 1998

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Interleukin-1 Type II Receptors
Interleukin-1 Type I Receptors
Ameloblasts
Tooth
Odontoblasts
Interleukin-1 Receptors
Messenger RNA
Interleukin-1
Amelogenesis
Dental Papilla
Dentinogenesis
Dental Sac
Dental Pulp
Reticulum
Osteoclasts
Dentin
Incisor
Dental Enamel
Crowns
Morphogenesis

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

Cite this

Expression of the mRNA for types I and II interleukin-1 receptors in dental tissues of mice during tooth development. / Xu, L. X.; Kukita, Toshio; Yu, H.; Nakano, Y.; Koga, T.

In: Calcified Tissue International, Vol. 63, No. 4, 01.10.1998, p. 351-356.

Research output: Contribution to journalArticle

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