Expression of the truncated E. coli O6-methylguanine methyltransferase gene in repair-deficient human cells and restoration of cellular resistance to alkylating agents

Kanji Ishizaki; Tohru Tsujimura; Chikau Fujio; Zhang Yangpei; Hideo Yawata; Yusaku Nakabeppu; Mutsuo Sekiguchi; Mituo Ikenaga

doi:10.1016/0167-8817(87)90068-X

Expression of the truncated E. coli O⁶-methylguanine methyltransferase gene in repair-deficient human cells and restoration of cellular resistance to alkylating agents

Kanji Ishizaki, Tohru Tsujimura, Chikau Fujio, Zhang Yangpei, Hideo Yawata, Yusaku Nakabeppu, Mutsuo Sekiguchi, Mituo Ikenaga

Department of Immunobiology and Neuroscience

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

We have constructed a truncated E. coli O⁶-methylguanine methyltransferase (MT) gene (ada gene) to express the MT activity for O⁶-methylguanine and O⁴-methylthymine but not for methylphosphotriester in human cells and transferred it into Mer^-HeLle MR cells. The transfectant cells expressed the truncated O⁶-E. coli MT were resistant to alkylating agents as same as the transfectant cells with the intact ada gene in cell killing, sister-chromatid exchange induction and host-cell reactivation of adenovirus 5. The results strongly suggest that methylphosphotriester may not contribute to the biological effect of alkylating agents in human cells.

Original language	English
Pages (from-to)	121-128
Number of pages	8
Journal	Mutation Research DNA Repair Reports
Volume	184
Issue number	2
DOIs	https://doi.org/10.1016/0167-8817(87)90068-X
Publication status	Published - Sept 1987

All Science Journal Classification (ASJC) codes

Medicine(all)

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10.1016/0167-8817(87)90068-X

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Ishizaki, K., Tsujimura, T., Fujio, C., Yangpei, Z., Yawata, H., Nakabeppu, Y., Sekiguchi, M., & Ikenaga, M. (1987). Expression of the truncated E. coli O⁶-methylguanine methyltransferase gene in repair-deficient human cells and restoration of cellular resistance to alkylating agents. Mutation Research DNA Repair Reports, 184(2), 121-128. https://doi.org/10.1016/0167-8817(87)90068-X

Expression of the truncated E. coli O⁶-methylguanine methyltransferase gene in repair-deficient human cells and restoration of cellular resistance to alkylating agents. / Ishizaki, Kanji; Tsujimura, Tohru; Fujio, Chikau et al.

In: Mutation Research DNA Repair Reports, Vol. 184, No. 2, 09.1987, p. 121-128.

Research output: Contribution to journal › Article › peer-review

Ishizaki, K, Tsujimura, T, Fujio, C, Yangpei, Z, Yawata, H, Nakabeppu, Y, Sekiguchi, M & Ikenaga, M 1987, 'Expression of the truncated E. coli O⁶-methylguanine methyltransferase gene in repair-deficient human cells and restoration of cellular resistance to alkylating agents', Mutation Research DNA Repair Reports, vol. 184, no. 2, pp. 121-128. https://doi.org/10.1016/0167-8817(87)90068-X

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abstract = "We have constructed a truncated E. coli O6-methylguanine methyltransferase (MT) gene (ada gene) to express the MT activity for O6-methylguanine and O4-methylthymine but not for methylphosphotriester in human cells and transferred it into Mer-HeLle MR cells. The transfectant cells expressed the truncated O6-E. coli MT were resistant to alkylating agents as same as the transfectant cells with the intact ada gene in cell killing, sister-chromatid exchange induction and host-cell reactivation of adenovirus 5. The results strongly suggest that methylphosphotriester may not contribute to the biological effect of alkylating agents in human cells.",

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Expression of the truncated E. coli O⁶-methylguanine methyltransferase gene in repair-deficient human cells and restoration of cellular resistance to alkylating agents

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