Expression of the type I collagen gene in rat periodontal ligament during tooth movement as revealed by in situ hybridization

Masahiro Nakagawa, Toshio Kukita, Akihiko Nakasima, Kojiro Kurisu

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The in situ hybridization technique used digoxigenin-labelled oligodeoxynucleotide. In untreated molars, cells expressing a positive signal for type I collagen mRNA were distributed uniformly in the periodontal ligament space. After experimental tooth movement, the density of cells expressing a positive signal appeared to be much greater in the tension side than the pressure side. In both sides the distribution of the positively hybridizing cells was uniform along the principal fibres of the ligament. This characteristic distribution appeared at 12 h after the initiation of tooth movement, reached a maximum at 1-3 days, and persisted for about 14 days during the treatment. These results indicate that the remodelling of collagen fibres in periodontal ligament occurs in an orderly manner throughout the principal fibres, mainly on the tension side, and that the recovery of gene expression for type I collagen occurs within the first 14 days in response to experimental tooth movement.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalArchives of Oral Biology
Volume39
Issue number4
DOIs
Publication statusPublished - Apr 1994

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this