Expression of toll-like receptor 2 on γδ T cells bearing invariant Vγ6/Vδ1 induced by Escherichia coli infection in mice

Yasuji Mokuno, Tetsuya Matsuguchi, Manabu Takano, Hitoshi Nishimura, Junji Washizu, Tomohiko Ogawa, Osamu Takeuchi, Shizuo Akira, Yuji Nimura, Yasunobu Yoshikai

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    124 Citations (Scopus)

    Abstract

    We recently reported that the number of γδ T cells was increased after infection with Escherichia coli in C3H/HeN mice. We here showed that an i.p. injection with native lipid A derived from E. coli induced an increase of γδ T cells in the peritoneal cavity of LPS-responsive C3H/HeN mice and, albeit to a lesser degree, also in LPS-hyporesponsive C3H/HeJ mice. The purified γδ T cells from C3H/HeN and C3H/HeJ mice expressed a canonical TCR repertoire encoded by Vγ6-Jγ1/Vδ1-Dδ2-Jδ2 gene segments and proliferated in response to the native lipid A derived from E. coli in a TCR-independent manner. The lipid A-reactive γδ T cells bearing canonical Vγ6/Vδ1 expressed Toll-like receptor (TLR) 2 mRNA, while TLR4 mRNA was undetectable. Treatment with a TLR2 anti-sense oligonucleotide resulted in hyporesponsiveness of the γδ T cells to the native lipid A. TLR2-deficient mice showed an impaired increase of the γδ T cells following injection of native lipid A. These results suggest that TLR2 is involved in the activation of canonical Vγ6/Vδ1 T cells by native E. coli lipid A.

    Original languageEnglish
    Pages (from-to)931-940
    Number of pages10
    JournalJournal of Immunology
    Volume165
    Issue number2
    DOIs
    Publication statusPublished - Jul 15 2000

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

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  • Cite this

    Mokuno, Y., Matsuguchi, T., Takano, M., Nishimura, H., Washizu, J., Ogawa, T., Takeuchi, O., Akira, S., Nimura, Y., & Yoshikai, Y. (2000). Expression of toll-like receptor 2 on γδ T cells bearing invariant Vγ6/Vδ1 induced by Escherichia coli infection in mice. Journal of Immunology, 165(2), 931-940. https://doi.org/10.4049/jimmunol.165.2.931