Expression of two kinds of cytochrome P-450(11β) mrna in bovine adrenal cortex

Shirou Kirita, Ken-Ichirou Morohashi, Toshihide Hashimoto, Hidefumi Yoshioka, Yoshiaki Fujii-kuriyama, Tsuneo Omura

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Using pcP-450(11β)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559-568) as the probe, a different cDNA clone, pcP-450(11β)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11β)-3 was highly homologous but not identical with that of pcP-450(11β)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11β)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11β) mRNA were expressed in individual animals and that a least two kinds of P-450(11β) genes exist in the bovine genome.

Original languageEnglish
Pages (from-to)683-686
Number of pages4
JournalJournal of biochemistry
Volume104
Issue number5
DOIs
Publication statusPublished - Jan 1 1988

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Adrenal Cortex
Cytochrome P-450 Enzyme System
Complementary DNA
Nucleotides
Genes
Oligonucleotide Probes
Gene Library
Sequence Analysis
Animals
Substitution reactions
Clone Cells
Genome
Amino Acids
Messenger RNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Expression of two kinds of cytochrome P-450(11β) mrna in bovine adrenal cortex. / Kirita, Shirou; Morohashi, Ken-Ichirou; Hashimoto, Toshihide; Yoshioka, Hidefumi; Fujii-kuriyama, Yoshiaki; Omura, Tsuneo.

In: Journal of biochemistry, Vol. 104, No. 5, 01.01.1988, p. 683-686.

Research output: Contribution to journalArticle

Kirita, Shirou ; Morohashi, Ken-Ichirou ; Hashimoto, Toshihide ; Yoshioka, Hidefumi ; Fujii-kuriyama, Yoshiaki ; Omura, Tsuneo. / Expression of two kinds of cytochrome P-450(11β) mrna in bovine adrenal cortex. In: Journal of biochemistry. 1988 ; Vol. 104, No. 5. pp. 683-686.
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