Expression of two kinds of cytochrome P-450(11β) mrna in bovine adrenal cortex

Shirou Kirita; Ken ichirou Morohashi; Toshihide Hashimoto; Hidefumi Yoshioka; Yoshiaki Fujii-kuriyama; Tsuneo Omura

doi:10.1093/oxfordjournals.jbchem.a122533

Expression of two kinds of cytochrome P-450(11β) mrna in bovine adrenal cortex

Shirou Kirita, Ken ichirou Morohashi, Toshihide Hashimoto, Hidefumi Yoshioka, Yoshiaki Fujii-kuriyama, Tsuneo Omura

Biology of Sex Difference

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Abstract

Using pcP-450(11β)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559-568) as the probe, a different cDNA clone, pcP-450(11β)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11β)-3 was highly homologous but not identical with that of pcP-450(11β)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11β)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11β) mRNA were expressed in individual animals and that a least two kinds of P-450(11β) genes exist in the bovine genome.

Original language	English
Pages (from-to)	683-686
Number of pages	4
Journal	Journal of biochemistry
Volume	104
Issue number	5
DOIs	https://doi.org/10.1093/oxfordjournals.jbchem.a122533
Publication status	Published - Nov 1988

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology

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10.1093/oxfordjournals.jbchem.a122533

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title = "Expression of two kinds of cytochrome P-450(11β) mrna in bovine adrenal cortex",

abstract = "Using pcP-450(11β)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559-568) as the probe, a different cDNA clone, pcP-450(11β)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11β)-3 was highly homologous but not identical with that of pcP-450(11β)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11β)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11β) mRNA were expressed in individual animals and that a least two kinds of P-450(11β) genes exist in the bovine genome.",

author = "Shirou Kirita and Morohashi, {Ken ichirou} and Toshihide Hashimoto and Hidefumi Yoshioka and Yoshiaki Fujii-kuriyama and Tsuneo Omura",

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T1 - Expression of two kinds of cytochrome P-450(11β) mrna in bovine adrenal cortex

AU - Kirita, Shirou

AU - Morohashi, Ken ichirou

AU - Hashimoto, Toshihide

AU - Yoshioka, Hidefumi

AU - Fujii-kuriyama, Yoshiaki

AU - Omura, Tsuneo

PY - 1988/11

Y1 - 1988/11

N2 - Using pcP-450(11β)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559-568) as the probe, a different cDNA clone, pcP-450(11β)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11β)-3 was highly homologous but not identical with that of pcP-450(11β)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11β)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11β) mRNA were expressed in individual animals and that a least two kinds of P-450(11β) genes exist in the bovine genome.

AB - Using pcP-450(11β)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559-568) as the probe, a different cDNA clone, pcP-450(11β)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11β)-3 was highly homologous but not identical with that of pcP-450(11β)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11β)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11β) mRNA were expressed in individual animals and that a least two kinds of P-450(11β) genes exist in the bovine genome.

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Expression of two kinds of cytochrome P-450(11β) mrna in bovine adrenal cortex

Abstract

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