Expression of uPAR mRNA in peripheral blood is a favourite marker for metastasis in gastric cancer cases

Y. Kita, T. Fukagawa, K. Mimori, Y. Kosaka, K. Ishikawa, T. Aikou, S. Natsugoe, M. Sasako, M. Mori

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Abstract

Urokinase-type plasminogen activator receptor (uPAR) plays a central role in the plasminogen activation cascade and participates in extracellular matrix degradation, cell migration and invasion. We evaluated the expression level of uPAR mRNA and the presence of isolated tumour cells (ITCs) in bone marrow (BM) and peripheral blood (PB) in gastric cancer patients and clarified its clinical significance. We assessed specific uPAR mRNA expression by quantitative real-time reverse transcriptase- polymerase chain reaction (RT-PCR) in BM and PB in 846 gastric cancer patients as well as three epithelial cell markers, carcinoembryonic antigen (CEA), cytokeratin (CK)-19 and CK-7. The uPAR mRNA expression in bone marrow and peripheral blood expressed significantly higher than normal controls (P<0.0001). The uPAR mRNA in BM showed concordant expression with the depth of tumour invasion, distant metastasis, and the postoperative recurrence (P=0.015, 0.044 and 0.010, respectively); whereas in PB, we observed more intimate significant association between uPAR expression and clinicopathologic variables, such as depth of tumour invasion, the distant metastasis, the venous invasion and the clinical stage (P=0.009, 0.002, 0.039 and 0.008, respectively). In addition, the uPAR mRNA expression in PB was an independent prognostic factor for distant metastasis by multivariate analysis. We disclosed that it was possible to identify high-risk patients for distant metastasis by measuring uPAR mRNA especially in peripheral blood at the timing of operation in gastric cancer patients.

Original languageEnglish
Pages (from-to)153-159
Number of pages7
JournalBritish journal of cancer
Volume100
Issue number1
DOIs
Publication statusPublished - Jan 13 2009

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Urokinase Plasminogen Activator Receptors
Stomach Neoplasms
Neoplasm Metastasis
Messenger RNA
Bone Marrow
Keratin-7
Keratin-19
Neoplasms
Plasminogen
Carcinoembryonic Antigen
Differentiation Antigens
Reverse Transcriptase Polymerase Chain Reaction
Bone Marrow Cells
Cell Movement
Extracellular Matrix
Real-Time Polymerase Chain Reaction
Multivariate Analysis
Epithelial Cells
Recurrence

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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Expression of uPAR mRNA in peripheral blood is a favourite marker for metastasis in gastric cancer cases. / Kita, Y.; Fukagawa, T.; Mimori, K.; Kosaka, Y.; Ishikawa, K.; Aikou, T.; Natsugoe, S.; Sasako, M.; Mori, M.

In: British journal of cancer, Vol. 100, No. 1, 13.01.2009, p. 153-159.

Research output: Contribution to journalArticle

Kita, Y. ; Fukagawa, T. ; Mimori, K. ; Kosaka, Y. ; Ishikawa, K. ; Aikou, T. ; Natsugoe, S. ; Sasako, M. ; Mori, M. / Expression of uPAR mRNA in peripheral blood is a favourite marker for metastasis in gastric cancer cases. In: British journal of cancer. 2009 ; Vol. 100, No. 1. pp. 153-159.
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