Expression of vascular endothelial growth factor in experimental choroidal neovascularization

Tatsuro Ishibashi, Yasuaki Hata, Hiroshi Yoshikawa, Kazunori Nakagawa, Katsuo Sueishi, Hajime Inomata

Research output: Contribution to journalArticle

213 Citations (Scopus)

Abstract

Background: Although the choroidal neovascularization (CNV) is a common pathologic feature of a number of different eye diseases, its pathological mechanisms have not been fully elucidated. We investigated the expression of vascular endothelial growth factor (VEGF) in CNV using an experimental primate model. Method: CNV was induced by intense laser photocoagulation in four monkey eyes. Single eyes were enucleated at 1, 3, 7 or 14 days after photocoagulation and examined immunohistochemically for VEGF, macrophage antigen, von Willebrand factor and glial fibrillary acidic protein (GFAP). Expression of VEGF mRNA was examined by in situ hybridization. Results: One day after photocoagulation, the normal structure of the outer portion of the retina and the inner portion of the choroid was destroyed. Three days after photocoagulation, choroidal vascular endothelial cells migrated into the subretinal space through the defect in Bruch's membrane. Increased expression of VEGF was detected in the accumulating macrophages, migrating retinal pigment epithelial (RPE) cells and Muller cells. Maximal expression of VEGF was observed between 3 and 7 days after wounding, and many newly formed vessels extended into the subretinal space 7-14 days after photocoagulation. Conclusion: VEGF derived from RPE cells, macrophages and Muller cells may play a role in the formation of CNV.

Original languageEnglish
Pages (from-to)159-167
Number of pages9
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume235
Issue number3
DOIs
Publication statusPublished - Apr 5 1997

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Choroidal Neovascularization
Light Coagulation
Vascular Endothelial Growth Factor A
Ependymoglial Cells
Retinal Pigments
Macrophages
Epithelial Cells
Bruch Membrane
Choroid
Eye Diseases
Glial Fibrillary Acidic Protein
von Willebrand Factor
Primates
Haplorhini
In Situ Hybridization
Retina
Lasers
Theoretical Models
Endothelial Cells
Antigens

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Expression of vascular endothelial growth factor in experimental choroidal neovascularization. / Ishibashi, Tatsuro; Hata, Yasuaki; Yoshikawa, Hiroshi; Nakagawa, Kazunori; Sueishi, Katsuo; Inomata, Hajime.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 235, No. 3, 05.04.1997, p. 159-167.

Research output: Contribution to journalArticle

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