Expression of vasohibin-1 in human carotid atherosclerotic plaque

Ryu Fukumitsu, Manabu Minami, Kazumichi Yoshida, Manabu Nagata, Mika Yasui, Sei Higuchi, Risako Fujikawa, Taichi Ikedo, Sen Yamagata, Yasufumi Sato, Hidenori Arai, Masayuki Yokode, Susumu Miyamoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aim: In patients with carotid plaque, intraplaque hemorrhage arising from ruptured neovascular vessels within the neointima is an important cause of stroke. The expression of Vasohibin-1 (VASH1), a negative feedback regulator of angiogenesis, occurs in the microvessel endothelial cells of various solid tumors and the arterial wall. However, the roles of VASH1 in the pathogenesis of atherosclerotic diseases remain unclear. The present study aimed to clarify the relevance of the VASH1 expression and plaque instability in human carotid plaques. Methods: We used quantitative real-time PCR and immunostaining to examine 12 atheromatous plaque specimens obtained via carotid endarterectomy. The distal areas of specimens lacking macroscopic atherosclerotic lesions served as controls. Results: Compared with that observed in the controls, the VASH1 gene expression increased significantly in the atheromatous plaque (p=0.018). Moreover, the VASH1 mRNA levels correlated positively with those of VEGFA, CD31 and VCAM1 (r =0.788, p=0.004; r =0.99, p<0.001; r =0.94, p< 0.001, respectively). Finally, the immunohistochemical analyses revealed the VASH1 expression in the neointimal microvessel endothelial cells of carotid plaque. Conclusions: The VASH1 expression levels in atheroma reflect both enhanced neovascularization and the inflammatory burden. Therefore, the VASH1 level may be a novel biomarker for evaluating plaque instability in patients with carotid arteriosclerosis and predicting ischemic stroke.

Original languageEnglish
Pages (from-to)942-948
Number of pages7
JournalJournal of atherosclerosis and thrombosis
Volume22
Issue number9
DOIs
Publication statusPublished - Sep 18 2015

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Endothelial cells
Atherosclerotic Plaques
Microvessels
Biomarkers
Endothelial Cells
Stroke
Gene expression
Tumors
Neointima
Carotid Endarterectomy
Arteriosclerosis
Feedback
Messenger RNA
Real-Time Polymerase Chain Reaction
Hemorrhage
Gene Expression
Neoplasms

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Cardiology and Cardiovascular Medicine
  • Biochemistry, medical

Cite this

Fukumitsu, R., Minami, M., Yoshida, K., Nagata, M., Yasui, M., Higuchi, S., ... Miyamoto, S. (2015). Expression of vasohibin-1 in human carotid atherosclerotic plaque. Journal of atherosclerosis and thrombosis, 22(9), 942-948. https://doi.org/10.5551/jat.29074

Expression of vasohibin-1 in human carotid atherosclerotic plaque. / Fukumitsu, Ryu; Minami, Manabu; Yoshida, Kazumichi; Nagata, Manabu; Yasui, Mika; Higuchi, Sei; Fujikawa, Risako; Ikedo, Taichi; Yamagata, Sen; Sato, Yasufumi; Arai, Hidenori; Yokode, Masayuki; Miyamoto, Susumu.

In: Journal of atherosclerosis and thrombosis, Vol. 22, No. 9, 18.09.2015, p. 942-948.

Research output: Contribution to journalArticle

Fukumitsu, R, Minami, M, Yoshida, K, Nagata, M, Yasui, M, Higuchi, S, Fujikawa, R, Ikedo, T, Yamagata, S, Sato, Y, Arai, H, Yokode, M & Miyamoto, S 2015, 'Expression of vasohibin-1 in human carotid atherosclerotic plaque', Journal of atherosclerosis and thrombosis, vol. 22, no. 9, pp. 942-948. https://doi.org/10.5551/jat.29074
Fukumitsu R, Minami M, Yoshida K, Nagata M, Yasui M, Higuchi S et al. Expression of vasohibin-1 in human carotid atherosclerotic plaque. Journal of atherosclerosis and thrombosis. 2015 Sep 18;22(9):942-948. https://doi.org/10.5551/jat.29074
Fukumitsu, Ryu ; Minami, Manabu ; Yoshida, Kazumichi ; Nagata, Manabu ; Yasui, Mika ; Higuchi, Sei ; Fujikawa, Risako ; Ikedo, Taichi ; Yamagata, Sen ; Sato, Yasufumi ; Arai, Hidenori ; Yokode, Masayuki ; Miyamoto, Susumu. / Expression of vasohibin-1 in human carotid atherosclerotic plaque. In: Journal of atherosclerosis and thrombosis. 2015 ; Vol. 22, No. 9. pp. 942-948.
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AU - Yoshida, Kazumichi

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AU - Higuchi, Sei

AU - Fujikawa, Risako

AU - Ikedo, Taichi

AU - Yamagata, Sen

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AU - Miyamoto, Susumu

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