Expression of zebrafish cyp11a1 as a maternal transcript and in yolk syncytial layer

Hwei Jan Hsu, Peihung Hsiao, Ming Wei Kuo, Bon Chu Chung

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Cyp11a1 (P450scc, cholesterol side-chain cleavage enzyme) is the first enzyme for the synthesis of all steroid hormones. The regulation of steroid synthesis has been extensively investigated, except during embryogenesis. To study steroidogenesis in embryos, we have isolated the zebrafish cyp11a1 gene, which consists of 11 exons. Reverse transcription-polymerase chain reaction analysis indicates that zebrafish cyp11a1 is expressed temporally in two waves during embryonic stages and when sexual differentiation begins. It is expressed in adult brain, testicular Leydig cells, and the granulosa/theca layer of the ovary. In addition, zebrafish cyp11a1 is expressed in oocytes, and is inherited as a maternal transcript in early embryos. Throughout zebrafish epiboly and segmentation stages, cyp11a1 is expressed in the yolk syncytial layer. At 36 h post fertilization, cyp11a1 transcript is located ventral to the third somite, where the primordial interrenal gland is located. In summary, zebrafish cyp11a1 is expressed in the cytoplasm of oocytes, as a maternal transcript, and in yolk syncytial layer during early embryogenesis.

Original languageEnglish
Pages (from-to)219-222
Number of pages4
JournalGene Expression Patterns
Volume2
Issue number3-4
DOIs
Publication statusPublished - Dec 1 2002

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Zebrafish
Mothers
Oocytes
Embryonic Development
Interrenal Gland
Embryonic Structures
Steroids
Cholesterol Side-Chain Cleavage Enzyme
Somites
Sex Differentiation
Leydig Cells
Fertilization
Reverse Transcription
Ovary
Exons
Cytoplasm
Hormones
Polymerase Chain Reaction
Brain
Enzymes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Developmental Biology

Cite this

Expression of zebrafish cyp11a1 as a maternal transcript and in yolk syncytial layer. / Hsu, Hwei Jan; Hsiao, Peihung; Kuo, Ming Wei; Chung, Bon Chu.

In: Gene Expression Patterns, Vol. 2, No. 3-4, 01.12.2002, p. 219-222.

Research output: Contribution to journalArticle

Hsu, Hwei Jan ; Hsiao, Peihung ; Kuo, Ming Wei ; Chung, Bon Chu. / Expression of zebrafish cyp11a1 as a maternal transcript and in yolk syncytial layer. In: Gene Expression Patterns. 2002 ; Vol. 2, No. 3-4. pp. 219-222.
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