Expression responses of the complement components in zebrafish organs after stimulation with poly I:C, mimicry of viral infection

Vo Kha Tam, Masakazu Tsujikura, Tomonori Somamoto, Miki Nakao

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Defensive roles of the complement system in fish are largely unknown, although expressions of several complement components have been examined in some teleost species upon bacterial and parasitic infections. To infer possible anti-virus defensive roles of the complement system in teleost, zebrafish were simulated by intraperitoneal injection of poly I: C, a double strand RNA analogue, to mimic viral infection, and expression of 24 complement components and their subunits in the gill, brain, kidney, gut, hepatopancreas, gonad, heart, spleen, and skin were analyzed by reverse-transcription PCR. In normal (unstimulated) fish, the hepatopancreas showed highest expression of most genes tested, and considerable extra hepatic expression was also observed for many complement components. Upon stimulation, the hepatopancreas showed decreased expression of CD11/CD18, B/C2-A3, MASP3, and properdin, while marked up-regulation of several complement component genes was observed in the gill, suggesting an important but unknown role of the gill in host defense against viral infection.

Original languageEnglish
Pages (from-to)389-395
Number of pages7
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume54
Issue number2
Publication statusPublished - Oct 1 2009

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Hepatopancreas
polyinosinic-polycytidylic acid
Poly I-C
Zebrafish
Virus Diseases
Danio rerio
complement
Fishes
hepatopancreas
Properdin
infection
Gene Components
gills
Parasitic Diseases
Gonads
Intraperitoneal Injections
Bacterial Infections
Reverse Transcription
Up-Regulation
Spleen

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

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abstract = "Defensive roles of the complement system in fish are largely unknown, although expressions of several complement components have been examined in some teleost species upon bacterial and parasitic infections. To infer possible anti-virus defensive roles of the complement system in teleost, zebrafish were simulated by intraperitoneal injection of poly I: C, a double strand RNA analogue, to mimic viral infection, and expression of 24 complement components and their subunits in the gill, brain, kidney, gut, hepatopancreas, gonad, heart, spleen, and skin were analyzed by reverse-transcription PCR. In normal (unstimulated) fish, the hepatopancreas showed highest expression of most genes tested, and considerable extra hepatic expression was also observed for many complement components. Upon stimulation, the hepatopancreas showed decreased expression of CD11/CD18, B/C2-A3, MASP3, and properdin, while marked up-regulation of several complement component genes was observed in the gill, suggesting an important but unknown role of the gill in host defense against viral infection.",
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AU - Tsujikura, Masakazu

AU - Somamoto, Tomonori

AU - Nakao, Miki

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