Extended blood half-life of monomethoxypolyethylene glycol-conjugated hen lysozyme is a key parameter controlling immunological tolerogenicity

T. So, H. O. Ito, M. Hirata, T. Ueda, T. Imoto

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The blood half-life of a protein is prolonged by conjugating a protein with a linear amphiphilic polymer, monomethoxypolyethylene glycol (mPEG). The conjugation gives a protein immunotolerogenicity; hence, it is likely that the long half-life is crucial for the tolerogenicity. We prepared a tolerogenic mPEG conjugate of hen egg lysozyme (mPEG1.5-HEL), which is conjugated 1.5-fold the molecular weight of mPEG against that of HEL, and evaluated the relationship between in vivo stability and the tolerogenicity. mPEG1.5-HEL retained immunogenicity to prime HEL-specific T cell and antibody responses and had a long blood half-life, more than 27 times that of native HEL. The tolerant state was maintained as long as mPEG1.5-HEL was detected in sera. With a decrease in the blood mPEG1.5-HEL level, the tolerant state returned gradually to the responsive state; however, reinjection of mPEG1.5-HEL again restored the tolerance. Thus, the extended blood half-life of HEL by mPEG conjugation is probably vital for establishing and maintaining the tolerant states.

Original languageEnglish
Pages (from-to)1187-1194
Number of pages8
JournalCellular and Molecular Life Sciences
Volume55
Issue number8-9
Publication statusPublished - Aug 11 1999

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Half-Life
Blood
Proteins
T-cells
Antibody Formation
Polymers
Molecular Weight
Molecular weight
T-Lymphocytes
monomethoxypolyethylene glycol
monomethoxypolyethylene glycol--conjugated hen lysozyme
Antibodies
Serum

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Extended blood half-life of monomethoxypolyethylene glycol-conjugated hen lysozyme is a key parameter controlling immunological tolerogenicity. / So, T.; Ito, H. O.; Hirata, M.; Ueda, T.; Imoto, T.

In: Cellular and Molecular Life Sciences, Vol. 55, No. 8-9, 11.08.1999, p. 1187-1194.

Research output: Contribution to journalArticle

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