Extension and measurements on a phospholipid vesicle by use of dual-beam optical tweezers

Masatoshi Ichikawa, Yoko Shitamichi, Yasuyuki Kimura

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A vesicle whose membrane is composed of a Phospholipid bilayer is called as a liposome. Since the fundamental structure of the membrane is the same as cell membranes, liposomes have been intensively studied as a simple model of live-cells. Here, we study the mechanical property of a micrometer-sized giant liposome through extending a single liposome from the inside by use of dual-beam optical tweezers. As the liposome is extended, the shape gradually changes from a sphere to a lemon-shape, and finally, a tubular membrane projection is exhibited. The surface tension and the bending rigidity of the lipid membrane are obtained from the measured force-extension curve. The present paper reviews both experimental and theoretical bases of this extension measurement method on the liposome with our experimental data.

Original languageEnglish
Title of host publication2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
Pages71-76
Number of pages6
DOIs
Publication statusPublished - Dec 1 2008
Event2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation" - Nagoya, Japan
Duration: Nov 6 2008Nov 9 2008

Publication series

Name2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008

Other

Other2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
CountryJapan
CityNagoya
Period11/6/0811/9/08

Fingerprint

Optical tweezers
Liposomes
Phospholipids
Membranes
Cell membranes
Rigidity
Surface tension
Mechanical properties

All Science Journal Classification (ASJC) codes

  • Computer Vision and Pattern Recognition
  • Human-Computer Interaction
  • Electrical and Electronic Engineering

Cite this

Ichikawa, M., Shitamichi, Y., & Kimura, Y. (2008). Extension and measurements on a phospholipid vesicle by use of dual-beam optical tweezers. In 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics" (pp. 71-76). [4752425] (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008). https://doi.org/10.1109/MHS.2008.4752425

Extension and measurements on a phospholipid vesicle by use of dual-beam optical tweezers. / Ichikawa, Masatoshi; Shitamichi, Yoko; Kimura, Yasuyuki.

2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics". 2008. p. 71-76 4752425 (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ichikawa, M, Shitamichi, Y & Kimura, Y 2008, Extension and measurements on a phospholipid vesicle by use of dual-beam optical tweezers. in 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"., 4752425, 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, pp. 71-76, 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation", Nagoya, Japan, 11/6/08. https://doi.org/10.1109/MHS.2008.4752425
Ichikawa M, Shitamichi Y, Kimura Y. Extension and measurements on a phospholipid vesicle by use of dual-beam optical tweezers. In 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics". 2008. p. 71-76. 4752425. (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008). https://doi.org/10.1109/MHS.2008.4752425
Ichikawa, Masatoshi ; Shitamichi, Yoko ; Kimura, Yasuyuki. / Extension and measurements on a phospholipid vesicle by use of dual-beam optical tweezers. 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics". 2008. pp. 71-76 (2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008).
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