Extension of a DNA molecule by local heating with a laser

Masatoshi Ichikawa, Hiroki Ichikawa, Kenichi Yoshikawa, Yasuyuki Kimura

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Thermal convection and thermophoresis induced by μm-scale local heating are shown to elongate a single DNA molecule. An infrared laser used as a point heat source is converged into a dispersion solution of DNA molecules, which is observed under a fluorescent microscope. The thermal convection around the laser focus manifests as extensional flow for the long DNA chain. A simulation of thermal convection that reproduces the experimental condition provides numerical support for the stretching caused by thermal convection. This DNA elongation technique is a novel method for manipulating the intact single DNA molecules, and it can be applied to a "lab on a chip".

Original languageEnglish
Article number148104
JournalPhysical review letters
Volume99
Issue number14
DOIs
Publication statusPublished - Oct 5 2007

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

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