Extensive lymphatic spread of cancer cells in patients with thoracic esophageal squamous cell carcinoma: Detection of CEA-mRNA in the three-field lymph nodes

Yu Imamura, Naoko Hayashi, Nobutaka Sato, Koichi Kinoshita, Junji Kurashige, Seiya Saito, Kotaro Hirashima, Ryuichi Karashima, Yukiharu Hiyoshi, Yohei Nagai, Masayuki Watanabe, Hideo Baba

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background and Objectives: The aim of this study is to clarify the extent of lymphatic spread of cancer cells using a novel genetic test to examine patients with thoracic esophageal squamous cell carcinoma (ESCC). Methods: A total of 35 patients who underwent an esophagectomy with three-field lymph node (LN) dissection were eligible. The regional LN stations were categorized into the cervical (C), recurrent nerve (RN), paraesophageal (PE), tracheo-bronchial (TB), and perigastric (PG) nodes. Lymphatic spread was pathologically diagnosed with Hematoxylin-Eosin (HE) and anti-cytokeratin immunohistochemistry (IHC) staining, and CEA-mRNA expression was examined using the transcription-reverse transcription concerted (TRC) reaction. Results: The rates of lymphatic spread with HE, IHC, and TRC were 7.2%, 10.1%, and 55.5%, respectively. The number of CEA-mRNA(+) LN stations significantly correlated with tumor depth, LN metastasis diagnosed by HE, and vascular invasions. CEA-mRNA expression was observed in 42.9%, 94.3%, 77.1%, 80.0%, and 82.9% of C, RN, TB, PE, and PG nodes, respectively. Conclusions: The high frequency of CEA-mRNA expression suggests that systemic therapy is necessary in addition to esophagectomy with adequate LN dissection. Conversely, a relatively low frequency of CEA-mRNA expression in the C node does not support the routine dissection of the LNs in this area.

Original languageEnglish
Pages (from-to)509-515
Number of pages7
JournalJournal of Surgical Oncology
Volume102
Issue number5
DOIs
Publication statusPublished - Oct 1 2010

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Thorax
Lymph Nodes
Hematoxylin
Eosine Yellowish-(YS)
Messenger RNA
Esophagectomy
Neoplasms
Lymph Node Excision
Immunohistochemistry
Keratins
Reverse Transcription
Blood Vessels
Dissection
Esophageal Squamous Cell Carcinoma
Staining and Labeling
Neoplasm Metastasis
Therapeutics

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

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Extensive lymphatic spread of cancer cells in patients with thoracic esophageal squamous cell carcinoma : Detection of CEA-mRNA in the three-field lymph nodes. / Imamura, Yu; Hayashi, Naoko; Sato, Nobutaka; Kinoshita, Koichi; Kurashige, Junji; Saito, Seiya; Hirashima, Kotaro; Karashima, Ryuichi; Hiyoshi, Yukiharu; Nagai, Yohei; Watanabe, Masayuki; Baba, Hideo.

In: Journal of Surgical Oncology, Vol. 102, No. 5, 01.10.2010, p. 509-515.

Research output: Contribution to journalArticle

Imamura, Y, Hayashi, N, Sato, N, Kinoshita, K, Kurashige, J, Saito, S, Hirashima, K, Karashima, R, Hiyoshi, Y, Nagai, Y, Watanabe, M & Baba, H 2010, 'Extensive lymphatic spread of cancer cells in patients with thoracic esophageal squamous cell carcinoma: Detection of CEA-mRNA in the three-field lymph nodes', Journal of Surgical Oncology, vol. 102, no. 5, pp. 509-515. https://doi.org/10.1002/jso.21621
Imamura, Yu ; Hayashi, Naoko ; Sato, Nobutaka ; Kinoshita, Koichi ; Kurashige, Junji ; Saito, Seiya ; Hirashima, Kotaro ; Karashima, Ryuichi ; Hiyoshi, Yukiharu ; Nagai, Yohei ; Watanabe, Masayuki ; Baba, Hideo. / Extensive lymphatic spread of cancer cells in patients with thoracic esophageal squamous cell carcinoma : Detection of CEA-mRNA in the three-field lymph nodes. In: Journal of Surgical Oncology. 2010 ; Vol. 102, No. 5. pp. 509-515.
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abstract = "Background and Objectives: The aim of this study is to clarify the extent of lymphatic spread of cancer cells using a novel genetic test to examine patients with thoracic esophageal squamous cell carcinoma (ESCC). Methods: A total of 35 patients who underwent an esophagectomy with three-field lymph node (LN) dissection were eligible. The regional LN stations were categorized into the cervical (C), recurrent nerve (RN), paraesophageal (PE), tracheo-bronchial (TB), and perigastric (PG) nodes. Lymphatic spread was pathologically diagnosed with Hematoxylin-Eosin (HE) and anti-cytokeratin immunohistochemistry (IHC) staining, and CEA-mRNA expression was examined using the transcription-reverse transcription concerted (TRC) reaction. Results: The rates of lymphatic spread with HE, IHC, and TRC were 7.2{\%}, 10.1{\%}, and 55.5{\%}, respectively. The number of CEA-mRNA(+) LN stations significantly correlated with tumor depth, LN metastasis diagnosed by HE, and vascular invasions. CEA-mRNA expression was observed in 42.9{\%}, 94.3{\%}, 77.1{\%}, 80.0{\%}, and 82.9{\%} of C, RN, TB, PE, and PG nodes, respectively. Conclusions: The high frequency of CEA-mRNA expression suggests that systemic therapy is necessary in addition to esophagectomy with adequate LN dissection. Conversely, a relatively low frequency of CEA-mRNA expression in the C node does not support the routine dissection of the LNs in this area.",
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AU - Kurashige, Junji

AU - Saito, Seiya

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