Extra-facile chiral separation of amino acid enantiomers by LC-TOFMS analysis

Yutaka Konya; Takeshi Bamba; Eiichiro Fukusaki

doi:10.1016/j.jbiosc.2015.06.017

Extra-facile chiral separation of amino acid enantiomers by LC-TOFMS analysis

Yutaka Konya, Takeshi Bamba, Eiichiro Fukusaki

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

An extra-facile chiral liquid chromatography-time of flight mass spectrometry (LC-TOFMS) analytical method of amino acid enantiomers has been developed without a derivatization process. The enantioseparation of eighteen proteinogenic amino acids (except for proline) was simultaneously performed using a combination of a chiral column (CROWNPAK CR-I(+)) and a TOFMS within 15 min. An isocratic condition of a simple mobile phase comprising acetonitrile/water/trifluoroacetic acid (96/4/0.5) gave baseline separation of all underivatized amino acid enantiomers on the chiral column.

Original language	English
Pages (from-to)	349-353
Number of pages	5
Journal	Journal of Bioscience and Bioengineering
Volume	121
Issue number	3
DOIs	https://doi.org/10.1016/j.jbiosc.2015.06.017
Publication status	Published - Mar 1 2016
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiosc.2015.06.017

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abstract = "An extra-facile chiral liquid chromatography-time of flight mass spectrometry (LC-TOFMS) analytical method of amino acid enantiomers has been developed without a derivatization process. The enantioseparation of eighteen proteinogenic amino acids (except for proline) was simultaneously performed using a combination of a chiral column (CROWNPAK CR-I(+)) and a TOFMS within 15 min. An isocratic condition of a simple mobile phase comprising acetonitrile/water/trifluoroacetic acid (96/4/0.5) gave baseline separation of all underivatized amino acid enantiomers on the chiral column.",

author = "Yutaka Konya and Takeshi Bamba and Eiichiro Fukusaki",

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AU - Bamba, Takeshi

AU - Fukusaki, Eiichiro

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Y1 - 2016/3/1

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AB - An extra-facile chiral liquid chromatography-time of flight mass spectrometry (LC-TOFMS) analytical method of amino acid enantiomers has been developed without a derivatization process. The enantioseparation of eighteen proteinogenic amino acids (except for proline) was simultaneously performed using a combination of a chiral column (CROWNPAK CR-I(+)) and a TOFMS within 15 min. An isocratic condition of a simple mobile phase comprising acetonitrile/water/trifluoroacetic acid (96/4/0.5) gave baseline separation of all underivatized amino acid enantiomers on the chiral column.

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ER -

Extra-facile chiral separation of amino acid enantiomers by LC-TOFMS analysis

Abstract

All Science Journal Classification (ASJC) codes

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