Extracellular adenosine 5′-triphosphate-evoked glutamate release in cultured hippocampal neurons

Kazuhide Inoue, Ken Nakazawa, Kannosuke Fujimori, Tomokazu Watano, Akira Takanaka

Research output: Contribution to journalArticle

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Abstract

Characteristics of extracellular ATP-evoked electrical responses in rat hippocampal neurons were investigated. Extracellular ATP (100 μM) induced a rapid depolarization followed by repetitive firings of spikes in these cells under whole-cell current-clamp. In whole-cell voltage-clamp experiments, ATP activated 2 types of inward currents that were inhibited by P2-purinoceptor blocker suramin (300 μM). One is a small (about -20 pA) sustained current which is insensitive to tetrodotoxin (TTX), and the other is a large (-100 to -300 pA) transient current which abolished in the presence of 3 μM TTX. The ATP-induced transient current was blocked by 6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX; 30 μM), a non-N-methyl-d-aspartate (non-NMDA) receptor antagonist. ATP failed to induce the transient current in the cell which showed the desensitization to quisqualic acid (QA; 10 μM), a non-NMDA receptor agonist. These findings suggest that ATP directly activates small sustained currents, and indirectly induces the transient currents by evoking glutamate release.

Original languageEnglish
Pages (from-to)215-218
Number of pages4
JournalNeuroscience Letters
Volume134
Issue number2
DOIs
Publication statusPublished - Jan 6 1992
Externally publishedYes

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Glutamic Acid
Adenosine Triphosphate
Neurons
Tetrodotoxin
Purinergic P2 Receptor Antagonists
Quisqualic Acid
6-Cyano-7-nitroquinoxaline-2,3-dione
Suramin
Quinoxalines
aspartic acid receptor

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Extracellular adenosine 5′-triphosphate-evoked glutamate release in cultured hippocampal neurons. / Inoue, Kazuhide; Nakazawa, Ken; Fujimori, Kannosuke; Watano, Tomokazu; Takanaka, Akira.

In: Neuroscience Letters, Vol. 134, No. 2, 06.01.1992, p. 215-218.

Research output: Contribution to journalArticle

Inoue, Kazuhide ; Nakazawa, Ken ; Fujimori, Kannosuke ; Watano, Tomokazu ; Takanaka, Akira. / Extracellular adenosine 5′-triphosphate-evoked glutamate release in cultured hippocampal neurons. In: Neuroscience Letters. 1992 ; Vol. 134, No. 2. pp. 215-218.
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