Extracellular adenosine 5′-triphosphate-evoked norepinephrine secretion not relating to voltage-gated Ca channels in pheochromocytoma PC12 cells

Kazuhide Inoue, Ken Nakazawa, Kannosuke Fujimori, Akira Takanaka

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

Characteristics of extracellular ATP-stimulated norepinephrine (NE) secretion were investigated by the batch method in relation to membrane current measured with the whole cell voltage-clamp technique. Extracellular ATP stimulated [3H]NE secretion from PC12 cells in a concentration range from 10 μM to 1 mM. The maximal effect of ATP was about 5 times larger than that of nicotine. ATP-stimulated secretion was extracellular Ca-dependent, but it was not inhibited by the Ca-channel blockers, cadmium (300 μM) or nicardipine (10 μM). Extracellular ATP activated an inward current in PC12 cells and the peak amplitude of the current was about 5 times larger than that activated by nicotine. These findings suggest that extracellular ATP activates receptor-operated channels and causes NE secretion.

Original languageEnglish
Pages (from-to)294-299
Number of pages6
JournalNeuroscience Letters
Volume106
Issue number3
DOIs
Publication statusPublished - Dec 4 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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