Extracellular secretion of phenolic substances from living brown algae

Toshiyuki Shibata, Yoichiro Hama, Taiko Miyasaki, Makoto Ito, Takashi Nakamura

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The chemical structures of ultraviolet (UV)- absorbing substances secreted from the healthy living brown algae, Eisenia bicyclis and Ecklonia kurome, were demonstrated. The living activity of algal cells was critically examined using a confocal laser-scanning microscope after incorporation of fluorescein diacetate (FDA) into the cells. Using thin-layer chromatography (TLC), reversed-phase three-dimensional high-performance liquid chromatography (RP-3D-HPLC) and gas chromatography-mass spectrometry (GC-MS), it was found that the UV-absorbing substances (λmax 265-270 nm) secreted from the living brown algae mainly consisted of the three monomeric bromophenols, 2,4-dibromophenol, 2,4,6-tribromophenol and dibromo-iodophenol, but not phloroglucinol or phlorotannins. The other minor compounds detected in the secretions were as follows: benzothiazole, fatty acids (14:1, 16:0 and 18:0 acids), franesol, 3-hydroxy-2,4,4-trimethylpentyl 2-methylpropanoate and squalene. Exudation of phloroglucinol and phlorotannins was ascertained to take place only after the cell death of these algae. These results indicate that, whilst the algae are alive, polymeric phlorotannins are strictly kept within the algal body, and only monomeric bromophenols are secreted into the seawater medium.

Original languageEnglish
Pages (from-to)787-794
Number of pages8
JournalJournal of Applied Phycology
Volume18
Issue number6
DOIs
Publication statusPublished - Dec 1 2006

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algae
Phaeophyceae
brown alga
secretion
Eisenia (Alariaceae)
phenolic compounds
high performance liquid chromatography
alga
squalene
exudation
fluorescein
thin layer chromatography
chemical structure
microscopes
chromatography
lasers
liquid chromatography
cell death
gas chromatography
fatty acid

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Plant Science

Cite this

Extracellular secretion of phenolic substances from living brown algae. / Shibata, Toshiyuki; Hama, Yoichiro; Miyasaki, Taiko; Ito, Makoto; Nakamura, Takashi.

In: Journal of Applied Phycology, Vol. 18, No. 6, 01.12.2006, p. 787-794.

Research output: Contribution to journalArticle

Shibata, T, Hama, Y, Miyasaki, T, Ito, M & Nakamura, T 2006, 'Extracellular secretion of phenolic substances from living brown algae', Journal of Applied Phycology, vol. 18, no. 6, pp. 787-794. https://doi.org/10.1007/s10811-006-9094-y
Shibata, Toshiyuki ; Hama, Yoichiro ; Miyasaki, Taiko ; Ito, Makoto ; Nakamura, Takashi. / Extracellular secretion of phenolic substances from living brown algae. In: Journal of Applied Phycology. 2006 ; Vol. 18, No. 6. pp. 787-794.
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